A subset of natural killer cells in peripheral blood displays a mature T cell phenotype

Normal human PBMC were analyzed for the presence of cells expressing both T3 and NKH1 antigens, using direct two-color immunofluorescence. In six individuals, NKH1+T3+ cells were found to represent 2.5% of PBMC and 24% of the total number of NKH1+ cells. Purified NKH1+T3+ cells were shown to have th...

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Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1986
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Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2188196/
https://www.ncbi.nlm.nih.gov/pubmed/3088199
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collection PubMed
description Normal human PBMC were analyzed for the presence of cells expressing both T3 and NKH1 antigens, using direct two-color immunofluorescence. In six individuals, NKH1+T3+ cells were found to represent 2.5% of PBMC and 24% of the total number of NKH1+ cells. Purified NKH1+T3+ cells were shown to have the typical morphology of large granular lymphocytes (LGL). NKH1+T3+ cells also exhibited spontaneous cytotoxicity against K562 target cells and this lytic activity could be inhibited by anti-T3 mAb. Similar results were obtained with NKH1+T3+ cells cultured in vitro in lymphocyte-conditioned medium. Taken together, these results indicate that NKH1+T3+ cells represent a unique population of NK-active cells in normal peripheral blood. Although these cells exhibit LGL morphology and NK activity, this appears to be mediated through a functional T cell-like receptor for target antigen.
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spelling pubmed-21881962008-04-17 A subset of natural killer cells in peripheral blood displays a mature T cell phenotype J Exp Med Articles Normal human PBMC were analyzed for the presence of cells expressing both T3 and NKH1 antigens, using direct two-color immunofluorescence. In six individuals, NKH1+T3+ cells were found to represent 2.5% of PBMC and 24% of the total number of NKH1+ cells. Purified NKH1+T3+ cells were shown to have the typical morphology of large granular lymphocytes (LGL). NKH1+T3+ cells also exhibited spontaneous cytotoxicity against K562 target cells and this lytic activity could be inhibited by anti-T3 mAb. Similar results were obtained with NKH1+T3+ cells cultured in vitro in lymphocyte-conditioned medium. Taken together, these results indicate that NKH1+T3+ cells represent a unique population of NK-active cells in normal peripheral blood. Although these cells exhibit LGL morphology and NK activity, this appears to be mediated through a functional T cell-like receptor for target antigen. The Rockefeller University Press 1986-07-01 /pmc/articles/PMC2188196/ /pubmed/3088199 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
A subset of natural killer cells in peripheral blood displays a mature T cell phenotype
title A subset of natural killer cells in peripheral blood displays a mature T cell phenotype
title_full A subset of natural killer cells in peripheral blood displays a mature T cell phenotype
title_fullStr A subset of natural killer cells in peripheral blood displays a mature T cell phenotype
title_full_unstemmed A subset of natural killer cells in peripheral blood displays a mature T cell phenotype
title_short A subset of natural killer cells in peripheral blood displays a mature T cell phenotype
title_sort subset of natural killer cells in peripheral blood displays a mature t cell phenotype
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2188196/
https://www.ncbi.nlm.nih.gov/pubmed/3088199

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