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MHC-restricted minimal regulatory circuit initiated by a class II- autoreactive T cell clone
The in vivo administration of a self-class II-reactive Th clone MS202 derived from C3H into syngeneic mice resulted in the suppression of both primary and early secondary antibody responses against T cell- dependent antigens. The suppression was due to the generation of antigen-nonspecific Ts cells...
Formato: | Texto |
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Lenguaje: | English |
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The Rockefeller University Press
1987
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2188308/ https://www.ncbi.nlm.nih.gov/pubmed/2952750 |
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collection | PubMed |
description | The in vivo administration of a self-class II-reactive Th clone MS202 derived from C3H into syngeneic mice resulted in the suppression of both primary and early secondary antibody responses against T cell- dependent antigens. The suppression was due to the generation of antigen-nonspecific Ts cells in the recipient, as the splenic T cells from the mice treated with MS202 were able to strongly suppress the in vitro secondary antibody response of primed syngeneic spleen cells. The dose-response curve of suppression indicated the generation of an effector type Ts that directly suppressed Th. The surface phenotype of Ts was Ly-1+,2-, L3T4+, I-J-. The presence of Ly-1+,2+ T cells was not required to induce the suppression. The suppression was strictly restricted to H-2k, as F1 Ts cells were able to suppress the response of C3H but not of B6 B cells helped by the same F1 Th cells. The experiments with chimeric mice indicated that the direct target of Ts is an MHC-restricted Th but not a B cell or APC. The results indicate the existence of a minimal regulatory circuit where an MHC-restricted Th induces a preprogrammed Ts that in turn directly suppresses Th with the same MHC-restriction specificity. The induction of and suppression by Ts appeared to be due to the direct recognition of MHC restriction sites of Th cells. |
format | Text |
id | pubmed-2188308 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1987 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21883082008-04-17 MHC-restricted minimal regulatory circuit initiated by a class II- autoreactive T cell clone J Exp Med Articles The in vivo administration of a self-class II-reactive Th clone MS202 derived from C3H into syngeneic mice resulted in the suppression of both primary and early secondary antibody responses against T cell- dependent antigens. The suppression was due to the generation of antigen-nonspecific Ts cells in the recipient, as the splenic T cells from the mice treated with MS202 were able to strongly suppress the in vitro secondary antibody response of primed syngeneic spleen cells. The dose-response curve of suppression indicated the generation of an effector type Ts that directly suppressed Th. The surface phenotype of Ts was Ly-1+,2-, L3T4+, I-J-. The presence of Ly-1+,2+ T cells was not required to induce the suppression. The suppression was strictly restricted to H-2k, as F1 Ts cells were able to suppress the response of C3H but not of B6 B cells helped by the same F1 Th cells. The experiments with chimeric mice indicated that the direct target of Ts is an MHC-restricted Th but not a B cell or APC. The results indicate the existence of a minimal regulatory circuit where an MHC-restricted Th induces a preprogrammed Ts that in turn directly suppresses Th with the same MHC-restriction specificity. The induction of and suppression by Ts appeared to be due to the direct recognition of MHC restriction sites of Th cells. The Rockefeller University Press 1987-05-01 /pmc/articles/PMC2188308/ /pubmed/2952750 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Articles MHC-restricted minimal regulatory circuit initiated by a class II- autoreactive T cell clone |
title | MHC-restricted minimal regulatory circuit initiated by a class II- autoreactive T cell clone |
title_full | MHC-restricted minimal regulatory circuit initiated by a class II- autoreactive T cell clone |
title_fullStr | MHC-restricted minimal regulatory circuit initiated by a class II- autoreactive T cell clone |
title_full_unstemmed | MHC-restricted minimal regulatory circuit initiated by a class II- autoreactive T cell clone |
title_short | MHC-restricted minimal regulatory circuit initiated by a class II- autoreactive T cell clone |
title_sort | mhc-restricted minimal regulatory circuit initiated by a class ii- autoreactive t cell clone |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2188308/ https://www.ncbi.nlm.nih.gov/pubmed/2952750 |