Oxygen-dependent modulation of erythropoietin mRNA levels in isolated rat kidneys studied by RNase protection

Using oligonucleotide primers complementary to conserved regions in the mouse erythropoietin (Epo) gene, a portion of the rat Epo gene was amplified by the polymerase chain reaction to produce a probe suitable for assay of rat Epo mRNA by RNAse protection. The assay, which has sufficient sensitivity...

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Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1990
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2188346/
https://www.ncbi.nlm.nih.gov/pubmed/2373996
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collection PubMed
description Using oligonucleotide primers complementary to conserved regions in the mouse erythropoietin (Epo) gene, a portion of the rat Epo gene was amplified by the polymerase chain reaction to produce a probe suitable for assay of rat Epo mRNA by RNAse protection. The assay, which has sufficient sensitivity to measure to Epo mRNA in unstimulated rat kidneys, was used to demonstrate high amplitude in vitro modulation of Epo mRNA levels in response to changes in perfusate flow rate and oxygen tension in isolated kidneys, thus providing clear evidence that all the necessary events linking changes in oxygen delivery to the modulation of Epo mRNA levels can occur intrarenally.
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spelling pubmed-21883462008-04-17 Oxygen-dependent modulation of erythropoietin mRNA levels in isolated rat kidneys studied by RNase protection J Exp Med Articles Using oligonucleotide primers complementary to conserved regions in the mouse erythropoietin (Epo) gene, a portion of the rat Epo gene was amplified by the polymerase chain reaction to produce a probe suitable for assay of rat Epo mRNA by RNAse protection. The assay, which has sufficient sensitivity to measure to Epo mRNA in unstimulated rat kidneys, was used to demonstrate high amplitude in vitro modulation of Epo mRNA levels in response to changes in perfusate flow rate and oxygen tension in isolated kidneys, thus providing clear evidence that all the necessary events linking changes in oxygen delivery to the modulation of Epo mRNA levels can occur intrarenally. The Rockefeller University Press 1990-08-01 /pmc/articles/PMC2188346/ /pubmed/2373996 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Oxygen-dependent modulation of erythropoietin mRNA levels in isolated rat kidneys studied by RNase protection
title Oxygen-dependent modulation of erythropoietin mRNA levels in isolated rat kidneys studied by RNase protection
title_full Oxygen-dependent modulation of erythropoietin mRNA levels in isolated rat kidneys studied by RNase protection
title_fullStr Oxygen-dependent modulation of erythropoietin mRNA levels in isolated rat kidneys studied by RNase protection
title_full_unstemmed Oxygen-dependent modulation of erythropoietin mRNA levels in isolated rat kidneys studied by RNase protection
title_short Oxygen-dependent modulation of erythropoietin mRNA levels in isolated rat kidneys studied by RNase protection
title_sort oxygen-dependent modulation of erythropoietin mrna levels in isolated rat kidneys studied by rnase protection
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2188346/
https://www.ncbi.nlm.nih.gov/pubmed/2373996

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