Cargando…
Cholesterol requirement of P3-X63-Ag8 and X63-Ag8.653 mouse myeloma cells for growth in vitro
P3-X63-Ag8 and X63-Ag8.653 mouse myeloma cells have an absolute requirement for cholesterol for growth under serum-free conditions. This requirement can be satisfied by low density lipoprotein at 2-6 micrograms/ml or by BSA-bound cholesterol at 5-10 micrograms/ml. Cholesterol-independent variants ca...
Formato: | Texto |
---|---|
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
1987
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2188360/ https://www.ncbi.nlm.nih.gov/pubmed/3585251 |
_version_ | 1782146390139338752 |
---|---|
collection | PubMed |
description | P3-X63-Ag8 and X63-Ag8.653 mouse myeloma cells have an absolute requirement for cholesterol for growth under serum-free conditions. This requirement can be satisfied by low density lipoprotein at 2-6 micrograms/ml or by BSA-bound cholesterol at 5-10 micrograms/ml. Cholesterol-independent variants can be selected after prolonged growth in low concentrations of serum. |
format | Text |
id | pubmed-2188360 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1987 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21883602008-04-17 Cholesterol requirement of P3-X63-Ag8 and X63-Ag8.653 mouse myeloma cells for growth in vitro J Exp Med Articles P3-X63-Ag8 and X63-Ag8.653 mouse myeloma cells have an absolute requirement for cholesterol for growth under serum-free conditions. This requirement can be satisfied by low density lipoprotein at 2-6 micrograms/ml or by BSA-bound cholesterol at 5-10 micrograms/ml. Cholesterol-independent variants can be selected after prolonged growth in low concentrations of serum. The Rockefeller University Press 1987-06-01 /pmc/articles/PMC2188360/ /pubmed/3585251 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Articles Cholesterol requirement of P3-X63-Ag8 and X63-Ag8.653 mouse myeloma cells for growth in vitro |
title | Cholesterol requirement of P3-X63-Ag8 and X63-Ag8.653 mouse myeloma cells for growth in vitro |
title_full | Cholesterol requirement of P3-X63-Ag8 and X63-Ag8.653 mouse myeloma cells for growth in vitro |
title_fullStr | Cholesterol requirement of P3-X63-Ag8 and X63-Ag8.653 mouse myeloma cells for growth in vitro |
title_full_unstemmed | Cholesterol requirement of P3-X63-Ag8 and X63-Ag8.653 mouse myeloma cells for growth in vitro |
title_short | Cholesterol requirement of P3-X63-Ag8 and X63-Ag8.653 mouse myeloma cells for growth in vitro |
title_sort | cholesterol requirement of p3-x63-ag8 and x63-ag8.653 mouse myeloma cells for growth in vitro |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2188360/ https://www.ncbi.nlm.nih.gov/pubmed/3585251 |