Production of a T cell hybridoma that expresses the T cell receptor gamma/delta heterodimer

We have produced a T cell hybridoma line by fusion of an IL-2- dependent, long-term T cell receptor (TCR) gamma/delta+ Thy-1+, bone marrow-derived, dendritic epidermal cell line to the BW5147 tumor line. The resultant hybridoma was rapidly growing, lymphokine independent, and expressed T3 in associa...

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Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1987
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2188374/
https://www.ncbi.nlm.nih.gov/pubmed/3108449
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collection PubMed
description We have produced a T cell hybridoma line by fusion of an IL-2- dependent, long-term T cell receptor (TCR) gamma/delta+ Thy-1+, bone marrow-derived, dendritic epidermal cell line to the BW5147 tumor line. The resultant hybridoma was rapidly growing, lymphokine independent, and expressed T3 in association with the TCR gamma/delta heterodimer. Several subclones of the hybridoma line produced easily detectable levels of IL-2 after stimulation by anti-T3 or Con A. The availability of these cloned cell lines should greatly facilitate further functional, biochemical, and molecular studies of the TCR delta chain.
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spelling pubmed-21883742008-04-17 Production of a T cell hybridoma that expresses the T cell receptor gamma/delta heterodimer J Exp Med Articles We have produced a T cell hybridoma line by fusion of an IL-2- dependent, long-term T cell receptor (TCR) gamma/delta+ Thy-1+, bone marrow-derived, dendritic epidermal cell line to the BW5147 tumor line. The resultant hybridoma was rapidly growing, lymphokine independent, and expressed T3 in association with the TCR gamma/delta heterodimer. Several subclones of the hybridoma line produced easily detectable levels of IL-2 after stimulation by anti-T3 or Con A. The availability of these cloned cell lines should greatly facilitate further functional, biochemical, and molecular studies of the TCR delta chain. The Rockefeller University Press 1987-06-01 /pmc/articles/PMC2188374/ /pubmed/3108449 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Production of a T cell hybridoma that expresses the T cell receptor gamma/delta heterodimer
title Production of a T cell hybridoma that expresses the T cell receptor gamma/delta heterodimer
title_full Production of a T cell hybridoma that expresses the T cell receptor gamma/delta heterodimer
title_fullStr Production of a T cell hybridoma that expresses the T cell receptor gamma/delta heterodimer
title_full_unstemmed Production of a T cell hybridoma that expresses the T cell receptor gamma/delta heterodimer
title_short Production of a T cell hybridoma that expresses the T cell receptor gamma/delta heterodimer
title_sort production of a t cell hybridoma that expresses the t cell receptor gamma/delta heterodimer
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2188374/
https://www.ncbi.nlm.nih.gov/pubmed/3108449

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