Early genetic events in T cell development analyzed by in situ hybridization

In situ hybridization was used to investigate the expression of T cell receptor (TCR) alpha, beta, and gamma mRNAs in developing fetal and adult precursor thymocytes. gamma transcription was observed at the earliest time tested (day 12), followed by beta 12 h later, and TCR alpha on day 16. The earl...

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Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1987
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2188376/
https://www.ncbi.nlm.nih.gov/pubmed/2884272
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collection PubMed
description In situ hybridization was used to investigate the expression of T cell receptor (TCR) alpha, beta, and gamma mRNAs in developing fetal and adult precursor thymocytes. gamma transcription was observed at the earliest time tested (day 12), followed by beta 12 h later, and TCR alpha on day 16. The early beta transcripts appeared to be from unrearranged or incompletely rearranged (D-J-C) beta loci. V beta region transcription was first detectable on day 14 and transcription of different V beta genes was induced at different times. These results delineate a schedule sequence of TCR gene activation, which begins within 1 d after entry of stem cells into the fetal thymus.
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spelling pubmed-21883762008-04-17 Early genetic events in T cell development analyzed by in situ hybridization J Exp Med Articles In situ hybridization was used to investigate the expression of T cell receptor (TCR) alpha, beta, and gamma mRNAs in developing fetal and adult precursor thymocytes. gamma transcription was observed at the earliest time tested (day 12), followed by beta 12 h later, and TCR alpha on day 16. The early beta transcripts appeared to be from unrearranged or incompletely rearranged (D-J-C) beta loci. V beta region transcription was first detectable on day 14 and transcription of different V beta genes was induced at different times. These results delineate a schedule sequence of TCR gene activation, which begins within 1 d after entry of stem cells into the fetal thymus. The Rockefeller University Press 1987-06-01 /pmc/articles/PMC2188376/ /pubmed/2884272 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Early genetic events in T cell development analyzed by in situ hybridization
title Early genetic events in T cell development analyzed by in situ hybridization
title_full Early genetic events in T cell development analyzed by in situ hybridization
title_fullStr Early genetic events in T cell development analyzed by in situ hybridization
title_full_unstemmed Early genetic events in T cell development analyzed by in situ hybridization
title_short Early genetic events in T cell development analyzed by in situ hybridization
title_sort early genetic events in t cell development analyzed by in situ hybridization
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2188376/
https://www.ncbi.nlm.nih.gov/pubmed/2884272

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