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Conversion of an M- group A streptococcus to M+ by transfer of a plasmid containing an M6 gene
An M28-derived group A streptococcal strain deleted for the gene encoding M protein was converted to M+ by introduction of a plasmid carrying emm6, the structural gene for type 6 M protein from strain D471. The reconstituted M+ strain, JRS2, resists phagocytosis in human blood and is opsonized by an...
Formato: | Texto |
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Lenguaje: | English |
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The Rockefeller University Press
1986
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2188471/ https://www.ncbi.nlm.nih.gov/pubmed/3534131 |
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collection | PubMed |
description | An M28-derived group A streptococcal strain deleted for the gene encoding M protein was converted to M+ by introduction of a plasmid carrying emm6, the structural gene for type 6 M protein from strain D471. The reconstituted M+ strain, JRS2, resists phagocytosis in human blood and is opsonized by anti-M6 hyperimmune serum, but not by anti- M28 serum. Immunofluorescent microscopy and ELISA demonstrate the presence of M protein on its surface. In addition, JRS2 removes opsonic antibodies from hyperimmune rabbit sera generated by immunization with purified ColiM6 protein and with a synthetic amino-terminal peptide derived from M6. Immunization of rabbits with JRS2 generates opsonic anti-M6 antibodies. These results indicate that the cloned emm6 gene contains the information necessary to convert a phagocytosis-sensitive streptococcus to phagocytosis resistance. Furthermore, it also contains the determinants for M type specificity and those required to elicit opsonic antibodies. It thus appears to determine all the traits associated with M protein. |
format | Text |
id | pubmed-2188471 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1986 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21884712008-04-17 Conversion of an M- group A streptococcus to M+ by transfer of a plasmid containing an M6 gene J Exp Med Articles An M28-derived group A streptococcal strain deleted for the gene encoding M protein was converted to M+ by introduction of a plasmid carrying emm6, the structural gene for type 6 M protein from strain D471. The reconstituted M+ strain, JRS2, resists phagocytosis in human blood and is opsonized by anti-M6 hyperimmune serum, but not by anti- M28 serum. Immunofluorescent microscopy and ELISA demonstrate the presence of M protein on its surface. In addition, JRS2 removes opsonic antibodies from hyperimmune rabbit sera generated by immunization with purified ColiM6 protein and with a synthetic amino-terminal peptide derived from M6. Immunization of rabbits with JRS2 generates opsonic anti-M6 antibodies. These results indicate that the cloned emm6 gene contains the information necessary to convert a phagocytosis-sensitive streptococcus to phagocytosis resistance. Furthermore, it also contains the determinants for M type specificity and those required to elicit opsonic antibodies. It thus appears to determine all the traits associated with M protein. The Rockefeller University Press 1986-11-01 /pmc/articles/PMC2188471/ /pubmed/3534131 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Articles Conversion of an M- group A streptococcus to M+ by transfer of a plasmid containing an M6 gene |
title | Conversion of an M- group A streptococcus to M+ by transfer of a plasmid containing an M6 gene |
title_full | Conversion of an M- group A streptococcus to M+ by transfer of a plasmid containing an M6 gene |
title_fullStr | Conversion of an M- group A streptococcus to M+ by transfer of a plasmid containing an M6 gene |
title_full_unstemmed | Conversion of an M- group A streptococcus to M+ by transfer of a plasmid containing an M6 gene |
title_short | Conversion of an M- group A streptococcus to M+ by transfer of a plasmid containing an M6 gene |
title_sort | conversion of an m- group a streptococcus to m+ by transfer of a plasmid containing an m6 gene |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2188471/ https://www.ncbi.nlm.nih.gov/pubmed/3534131 |