A murine hybridoma with large cytoplasmic inclusions of kappa light chains

A murine hybridoma cell line has been established that consistently forms large cytoplasmic inclusions. These structures bind antibody specific for mouse kappa L chain when stained in situ. SDS-PAGE analysis of isolated inclusion bodies produce a single protein band of approximately 26,000 Mr that r...

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Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1987
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2188503/
https://www.ncbi.nlm.nih.gov/pubmed/3029271
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collection PubMed
description A murine hybridoma cell line has been established that consistently forms large cytoplasmic inclusions. These structures bind antibody specific for mouse kappa L chain when stained in situ. SDS-PAGE analysis of isolated inclusion bodies produce a single protein band of approximately 26,000 Mr that reacts with anti-kappa antibody when transferred to nitrocellulose. No carbohydrate was detected in association with the purified protein. These data are consistent with the intracellular retention and deposition of complete kappa L chain protein.
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spelling pubmed-21885032008-04-17 A murine hybridoma with large cytoplasmic inclusions of kappa light chains J Exp Med Articles A murine hybridoma cell line has been established that consistently forms large cytoplasmic inclusions. These structures bind antibody specific for mouse kappa L chain when stained in situ. SDS-PAGE analysis of isolated inclusion bodies produce a single protein band of approximately 26,000 Mr that reacts with anti-kappa antibody when transferred to nitrocellulose. No carbohydrate was detected in association with the purified protein. These data are consistent with the intracellular retention and deposition of complete kappa L chain protein. The Rockefeller University Press 1987-02-01 /pmc/articles/PMC2188503/ /pubmed/3029271 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
A murine hybridoma with large cytoplasmic inclusions of kappa light chains
title A murine hybridoma with large cytoplasmic inclusions of kappa light chains
title_full A murine hybridoma with large cytoplasmic inclusions of kappa light chains
title_fullStr A murine hybridoma with large cytoplasmic inclusions of kappa light chains
title_full_unstemmed A murine hybridoma with large cytoplasmic inclusions of kappa light chains
title_short A murine hybridoma with large cytoplasmic inclusions of kappa light chains
title_sort murine hybridoma with large cytoplasmic inclusions of kappa light chains
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2188503/
https://www.ncbi.nlm.nih.gov/pubmed/3029271

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