Cloning and characterization of cDNAs for murine macrophage inflammatory protein 2 and its human homologues

A cDNA clone of murine macrophage inflammatory protein 2 (MIP-2) has been isolated from a library prepared from lipopolysaccharide (LPS)- stimulated RAW 264.7 cells and the nucleotide sequence determined. This cDNA was used to clone cDNAs for human homologues of MIP-2 from a library prepared from ph...

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Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1990
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2188540/
https://www.ncbi.nlm.nih.gov/pubmed/2201751
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collection PubMed
description A cDNA clone of murine macrophage inflammatory protein 2 (MIP-2) has been isolated from a library prepared from lipopolysaccharide (LPS)- stimulated RAW 264.7 cells and the nucleotide sequence determined. This cDNA was used to clone cDNAs for human homologues of MIP-2 from a library prepared from phorbol myristate acetate-treated and LPS- stimulated U937 cells. Two homologues were isolated and sequenced. Human MIP-2 alpha and MIP-2 beta are highly homologous to each other and to a previously isolated gene, human gro/melanoma growth- stimulating activity (MGSA). These three human genes, MIP-2 alpha, MIP- 2 beta, and gro/MGSA, constitute a sub-family within the cytokine family represented by platelet factor 4 and interleukin 8.
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spelling pubmed-21885402008-04-17 Cloning and characterization of cDNAs for murine macrophage inflammatory protein 2 and its human homologues J Exp Med Articles A cDNA clone of murine macrophage inflammatory protein 2 (MIP-2) has been isolated from a library prepared from lipopolysaccharide (LPS)- stimulated RAW 264.7 cells and the nucleotide sequence determined. This cDNA was used to clone cDNAs for human homologues of MIP-2 from a library prepared from phorbol myristate acetate-treated and LPS- stimulated U937 cells. Two homologues were isolated and sequenced. Human MIP-2 alpha and MIP-2 beta are highly homologous to each other and to a previously isolated gene, human gro/melanoma growth- stimulating activity (MGSA). These three human genes, MIP-2 alpha, MIP- 2 beta, and gro/MGSA, constitute a sub-family within the cytokine family represented by platelet factor 4 and interleukin 8. The Rockefeller University Press 1990-09-01 /pmc/articles/PMC2188540/ /pubmed/2201751 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Cloning and characterization of cDNAs for murine macrophage inflammatory protein 2 and its human homologues
title Cloning and characterization of cDNAs for murine macrophage inflammatory protein 2 and its human homologues
title_full Cloning and characterization of cDNAs for murine macrophage inflammatory protein 2 and its human homologues
title_fullStr Cloning and characterization of cDNAs for murine macrophage inflammatory protein 2 and its human homologues
title_full_unstemmed Cloning and characterization of cDNAs for murine macrophage inflammatory protein 2 and its human homologues
title_short Cloning and characterization of cDNAs for murine macrophage inflammatory protein 2 and its human homologues
title_sort cloning and characterization of cdnas for murine macrophage inflammatory protein 2 and its human homologues
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2188540/
https://www.ncbi.nlm.nih.gov/pubmed/2201751

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