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A tissue-specific transcriptional enhancer is found in the body of the HLA-DR alpha gene
We mapped cis-acting regulatory elements in the HLA-DR alpha gene, which encodes the monomorphic subunit of the HLA-DR heterodimer. Genomic fragments of HLA-DR alpha were placed 5' or 3' to the chloramphenicol acetyltransferase reporter gene, the transcription of which was initiated from t...
Formato: | Texto |
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Lenguaje: | English |
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The Rockefeller University Press
1987
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2188692/ https://www.ncbi.nlm.nih.gov/pubmed/3476684 |
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collection | PubMed |
description | We mapped cis-acting regulatory elements in the HLA-DR alpha gene, which encodes the monomorphic subunit of the HLA-DR heterodimer. Genomic fragments of HLA-DR alpha were placed 5' or 3' to the chloramphenicol acetyltransferase reporter gene, the transcription of which was initiated from the Herpes simplex thymidine kinase promoter. In transient expression assays, fragments from the body of the HLA-DR alpha gene were able to increase chloramphenicol acetyltransferase activity in a position-, orientation-, and promoter-independent yet tissue-specific fashion. These HLA-DR alpha cis-acting regulatory elements contain previously identified DNase I-hypersensitive sites and DNA sequences homologous to those found in other eukaryotic transcriptional enhancers. |
format | Text |
id | pubmed-2188692 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1987 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21886922008-04-17 A tissue-specific transcriptional enhancer is found in the body of the HLA-DR alpha gene J Exp Med Articles We mapped cis-acting regulatory elements in the HLA-DR alpha gene, which encodes the monomorphic subunit of the HLA-DR heterodimer. Genomic fragments of HLA-DR alpha were placed 5' or 3' to the chloramphenicol acetyltransferase reporter gene, the transcription of which was initiated from the Herpes simplex thymidine kinase promoter. In transient expression assays, fragments from the body of the HLA-DR alpha gene were able to increase chloramphenicol acetyltransferase activity in a position-, orientation-, and promoter-independent yet tissue-specific fashion. These HLA-DR alpha cis-acting regulatory elements contain previously identified DNase I-hypersensitive sites and DNA sequences homologous to those found in other eukaryotic transcriptional enhancers. The Rockefeller University Press 1987-09-01 /pmc/articles/PMC2188692/ /pubmed/3476684 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Articles A tissue-specific transcriptional enhancer is found in the body of the HLA-DR alpha gene |
title | A tissue-specific transcriptional enhancer is found in the body of the HLA-DR alpha gene |
title_full | A tissue-specific transcriptional enhancer is found in the body of the HLA-DR alpha gene |
title_fullStr | A tissue-specific transcriptional enhancer is found in the body of the HLA-DR alpha gene |
title_full_unstemmed | A tissue-specific transcriptional enhancer is found in the body of the HLA-DR alpha gene |
title_short | A tissue-specific transcriptional enhancer is found in the body of the HLA-DR alpha gene |
title_sort | tissue-specific transcriptional enhancer is found in the body of the hla-dr alpha gene |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2188692/ https://www.ncbi.nlm.nih.gov/pubmed/3476684 |