Sequence analysis of cDNA coding for a major house dust mite allergen, Der p 1. Homology with cysteine proteases

A cDNA clone coding for Der p 1, a major allergen from the house dust mite Dermatophagoides pteronyssinus, has been sequenced. It codes for a 222 residue mature protein with a derived molecular weight of 25,371 and contains 1 potential N-glycosylation site. In addition, the cDNA appears to code for...

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Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1988
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2188807/
https://www.ncbi.nlm.nih.gov/pubmed/3335830
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description A cDNA clone coding for Der p 1, a major allergen from the house dust mite Dermatophagoides pteronyssinus, has been sequenced. It codes for a 222 residue mature protein with a derived molecular weight of 25,371 and contains 1 potential N-glycosylation site. In addition, the cDNA appears to code for a 13 residue proregion, and an incomplete signal peptide. The deduced sequence shows a high degree of homology with animal and plant cysteine proteases, particularly in the region of the contact residues making up the active site. Southern analysis of genomic DNA indicates that the allergen is coded by a noncontiguous gene. These data will now facilitate epitope mapping studies.
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spelling pubmed-21888072008-04-17 Sequence analysis of cDNA coding for a major house dust mite allergen, Der p 1. Homology with cysteine proteases J Exp Med Articles A cDNA clone coding for Der p 1, a major allergen from the house dust mite Dermatophagoides pteronyssinus, has been sequenced. It codes for a 222 residue mature protein with a derived molecular weight of 25,371 and contains 1 potential N-glycosylation site. In addition, the cDNA appears to code for a 13 residue proregion, and an incomplete signal peptide. The deduced sequence shows a high degree of homology with animal and plant cysteine proteases, particularly in the region of the contact residues making up the active site. Southern analysis of genomic DNA indicates that the allergen is coded by a noncontiguous gene. These data will now facilitate epitope mapping studies. The Rockefeller University Press 1988-01-01 /pmc/articles/PMC2188807/ /pubmed/3335830 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Sequence analysis of cDNA coding for a major house dust mite allergen, Der p 1. Homology with cysteine proteases
title Sequence analysis of cDNA coding for a major house dust mite allergen, Der p 1. Homology with cysteine proteases
title_full Sequence analysis of cDNA coding for a major house dust mite allergen, Der p 1. Homology with cysteine proteases
title_fullStr Sequence analysis of cDNA coding for a major house dust mite allergen, Der p 1. Homology with cysteine proteases
title_full_unstemmed Sequence analysis of cDNA coding for a major house dust mite allergen, Der p 1. Homology with cysteine proteases
title_short Sequence analysis of cDNA coding for a major house dust mite allergen, Der p 1. Homology with cysteine proteases
title_sort sequence analysis of cdna coding for a major house dust mite allergen, der p 1. homology with cysteine proteases
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2188807/
https://www.ncbi.nlm.nih.gov/pubmed/3335830

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