Acquisition of an additional antigen specificity after mouse CD4 gene transfer into a T helper hybridoma

We have transfected the mouse CD4 gene into a beef insulin (BI)- specific murine T helper hybridoma that lacks CD4 surface expression. The CD4-expressing transfectants have acquired an additional reactivity for pork insulin (PI), which was not detectable in the original recipient cell. The transfect...

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Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1988
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2188913/
https://www.ncbi.nlm.nih.gov/pubmed/2965740
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collection PubMed
description We have transfected the mouse CD4 gene into a beef insulin (BI)- specific murine T helper hybridoma that lacks CD4 surface expression. The CD4-expressing transfectants have acquired an additional reactivity for pork insulin (PI), which was not detectable in the original recipient cell. The transfectants' response to PI can be completely abrogated by anti-CD4 antibodies. The transfected clone showed a 50- fold increased sensitivity towards BI in comparison to the same CD4- hybridoma. These experiments suggest that CD4 may be important in determining the antigen fine specificity and, therefore, may also play a role in altering the T cell repertoire.
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spelling pubmed-21889132008-04-17 Acquisition of an additional antigen specificity after mouse CD4 gene transfer into a T helper hybridoma J Exp Med Articles We have transfected the mouse CD4 gene into a beef insulin (BI)- specific murine T helper hybridoma that lacks CD4 surface expression. The CD4-expressing transfectants have acquired an additional reactivity for pork insulin (PI), which was not detectable in the original recipient cell. The transfectants' response to PI can be completely abrogated by anti-CD4 antibodies. The transfected clone showed a 50- fold increased sensitivity towards BI in comparison to the same CD4- hybridoma. These experiments suggest that CD4 may be important in determining the antigen fine specificity and, therefore, may also play a role in altering the T cell repertoire. The Rockefeller University Press 1988-04-01 /pmc/articles/PMC2188913/ /pubmed/2965740 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Acquisition of an additional antigen specificity after mouse CD4 gene transfer into a T helper hybridoma
title Acquisition of an additional antigen specificity after mouse CD4 gene transfer into a T helper hybridoma
title_full Acquisition of an additional antigen specificity after mouse CD4 gene transfer into a T helper hybridoma
title_fullStr Acquisition of an additional antigen specificity after mouse CD4 gene transfer into a T helper hybridoma
title_full_unstemmed Acquisition of an additional antigen specificity after mouse CD4 gene transfer into a T helper hybridoma
title_short Acquisition of an additional antigen specificity after mouse CD4 gene transfer into a T helper hybridoma
title_sort acquisition of an additional antigen specificity after mouse cd4 gene transfer into a t helper hybridoma
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2188913/
https://www.ncbi.nlm.nih.gov/pubmed/2965740

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