VH gene family utilization is regulated by a locus outside of the VH region

We have used the RNA colony blot method to examine VH usage in colonies derived from primary splenic B cells. We found that there are strain- specific differences in the pattern of VH usage. Using parental F1, congenic, and recombinant inbred strains we demonstrate that the genetic element that caus...

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Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1988
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2188925/
https://www.ncbi.nlm.nih.gov/pubmed/2451707
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description We have used the RNA colony blot method to examine VH usage in colonies derived from primary splenic B cells. We found that there are strain- specific differences in the pattern of VH usage. Using parental F1, congenic, and recombinant inbred strains we demonstrate that the genetic element that causes the observed phenotype is: (a) stably expressed; (b) not due to maternal influence; (c) not due to dominate diffusible factors; (d) not linked to cloning efficiency; and (e) outside the Igh locus.
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spelling pubmed-21889252008-04-17 VH gene family utilization is regulated by a locus outside of the VH region J Exp Med Articles We have used the RNA colony blot method to examine VH usage in colonies derived from primary splenic B cells. We found that there are strain- specific differences in the pattern of VH usage. Using parental F1, congenic, and recombinant inbred strains we demonstrate that the genetic element that causes the observed phenotype is: (a) stably expressed; (b) not due to maternal influence; (c) not due to dominate diffusible factors; (d) not linked to cloning efficiency; and (e) outside the Igh locus. The Rockefeller University Press 1988-04-01 /pmc/articles/PMC2188925/ /pubmed/2451707 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
VH gene family utilization is regulated by a locus outside of the VH region
title VH gene family utilization is regulated by a locus outside of the VH region
title_full VH gene family utilization is regulated by a locus outside of the VH region
title_fullStr VH gene family utilization is regulated by a locus outside of the VH region
title_full_unstemmed VH gene family utilization is regulated by a locus outside of the VH region
title_short VH gene family utilization is regulated by a locus outside of the VH region
title_sort vh gene family utilization is regulated by a locus outside of the vh region
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2188925/
https://www.ncbi.nlm.nih.gov/pubmed/2451707

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