Anti-liver kidney microsome antibody recognizes a cytochrome P450 from the IID subfamily

Children with autoimmune hepatitis have high serum titers of antibodies directed against a 50-kD protein of rat liver endoplasmic reticulum. Affinity-purified anti-50-kD antibodies were used to screen a rat liver cDNA library in lambda GT-11 expression vector. 12 immunopositive clones were obtained....

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Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1988
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2189003/
https://www.ncbi.nlm.nih.gov/pubmed/2842431
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collection PubMed
description Children with autoimmune hepatitis have high serum titers of antibodies directed against a 50-kD protein of rat liver endoplasmic reticulum. Affinity-purified anti-50-kD antibodies were used to screen a rat liver cDNA library in lambda GT-11 expression vector. 12 immunopositive clones were obtained. Crossreactivities between fusion proteins of these clones and the 50-kD protein was demonstrated, and four clones were analyzed by restriction mapping, one of them by nucleotide sequencing. Complete identity was found between the restriction maps of two clones (LKMC1 and LKMC2) and that of the 5' end of the rat cytochrome P450 db2. Sequence of a 608-bp fragment of LKMC1 showed complete homology with the rat P450 db2 form. The restriction map of the other two clones (LKMC3 and LKMC4) was identical to that of rat P450 db1. These results suggest that the antigen recognized by LKMA is a P450 of the IID subfamily.
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spelling pubmed-21890032008-04-17 Anti-liver kidney microsome antibody recognizes a cytochrome P450 from the IID subfamily J Exp Med Articles Children with autoimmune hepatitis have high serum titers of antibodies directed against a 50-kD protein of rat liver endoplasmic reticulum. Affinity-purified anti-50-kD antibodies were used to screen a rat liver cDNA library in lambda GT-11 expression vector. 12 immunopositive clones were obtained. Crossreactivities between fusion proteins of these clones and the 50-kD protein was demonstrated, and four clones were analyzed by restriction mapping, one of them by nucleotide sequencing. Complete identity was found between the restriction maps of two clones (LKMC1 and LKMC2) and that of the 5' end of the rat cytochrome P450 db2. Sequence of a 608-bp fragment of LKMC1 showed complete homology with the rat P450 db2 form. The restriction map of the other two clones (LKMC3 and LKMC4) was identical to that of rat P450 db1. These results suggest that the antigen recognized by LKMA is a P450 of the IID subfamily. The Rockefeller University Press 1988-08-01 /pmc/articles/PMC2189003/ /pubmed/2842431 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Anti-liver kidney microsome antibody recognizes a cytochrome P450 from the IID subfamily
title Anti-liver kidney microsome antibody recognizes a cytochrome P450 from the IID subfamily
title_full Anti-liver kidney microsome antibody recognizes a cytochrome P450 from the IID subfamily
title_fullStr Anti-liver kidney microsome antibody recognizes a cytochrome P450 from the IID subfamily
title_full_unstemmed Anti-liver kidney microsome antibody recognizes a cytochrome P450 from the IID subfamily
title_short Anti-liver kidney microsome antibody recognizes a cytochrome P450 from the IID subfamily
title_sort anti-liver kidney microsome antibody recognizes a cytochrome p450 from the iid subfamily
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2189003/
https://www.ncbi.nlm.nih.gov/pubmed/2842431

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