Isolation and immunocytochemical characterization of human bone marrow stromal macrophages in hemopoietic clusters

Stromal macrophages (M phi) have been localized in situ and isolated within erythroid clusters from human marrow. Stromal M phi arborize in an extensive network uniformly distributed throughout marrow interstitium, and express the phenotype CD4+, CD11a+, CD11c+, CD13+, CD14+, CD16+, CD18+, CD31+, CD...

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Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1988
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2189021/
https://www.ncbi.nlm.nih.gov/pubmed/3049905
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description Stromal macrophages (M phi) have been localized in situ and isolated within erythroid clusters from human marrow. Stromal M phi arborize in an extensive network uniformly distributed throughout marrow interstitium, and express the phenotype CD4+, CD11a+, CD11c+, CD13+, CD14+, CD16+, CD18+, CD31+, CD32+, FcRI+, HLA-DR+, and CD35-, transferrin receptor-negative, and CD11b (weak). They express endocytic receptor antigens, but show significant differences in myeloid antigen expression compared with freshly harvested or cultured monocytes. Human stromal M phi are therefore specialized mature marrow M phi that are accessible for further investigations in infectious, storage, or hemopoietic disorders.
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spelling pubmed-21890212008-04-17 Isolation and immunocytochemical characterization of human bone marrow stromal macrophages in hemopoietic clusters J Exp Med Articles Stromal macrophages (M phi) have been localized in situ and isolated within erythroid clusters from human marrow. Stromal M phi arborize in an extensive network uniformly distributed throughout marrow interstitium, and express the phenotype CD4+, CD11a+, CD11c+, CD13+, CD14+, CD16+, CD18+, CD31+, CD32+, FcRI+, HLA-DR+, and CD35-, transferrin receptor-negative, and CD11b (weak). They express endocytic receptor antigens, but show significant differences in myeloid antigen expression compared with freshly harvested or cultured monocytes. Human stromal M phi are therefore specialized mature marrow M phi that are accessible for further investigations in infectious, storage, or hemopoietic disorders. The Rockefeller University Press 1988-09-01 /pmc/articles/PMC2189021/ /pubmed/3049905 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Isolation and immunocytochemical characterization of human bone marrow stromal macrophages in hemopoietic clusters
title Isolation and immunocytochemical characterization of human bone marrow stromal macrophages in hemopoietic clusters
title_full Isolation and immunocytochemical characterization of human bone marrow stromal macrophages in hemopoietic clusters
title_fullStr Isolation and immunocytochemical characterization of human bone marrow stromal macrophages in hemopoietic clusters
title_full_unstemmed Isolation and immunocytochemical characterization of human bone marrow stromal macrophages in hemopoietic clusters
title_short Isolation and immunocytochemical characterization of human bone marrow stromal macrophages in hemopoietic clusters
title_sort isolation and immunocytochemical characterization of human bone marrow stromal macrophages in hemopoietic clusters
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2189021/
https://www.ncbi.nlm.nih.gov/pubmed/3049905

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