Cargando…

Properties of isolated red pulp macrophages from mouse spleen

Dense monolayers of large, adherent macrophages were prepared from the red pulp of mouse spleen. These sinus-lining phagocytes resembled liver Kupffer cells in morphology, as well as expression of F4/80 and class II MHC antigens and receptors for IgG. C3-coated red cells attached at low levels to sp...

Descripción completa

Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1988
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2189070/
https://www.ncbi.nlm.nih.gov/pubmed/2971759
_version_ 1782146556139405312
collection PubMed
description Dense monolayers of large, adherent macrophages were prepared from the red pulp of mouse spleen. These sinus-lining phagocytes resembled liver Kupffer cells in morphology, as well as expression of F4/80 and class II MHC antigens and receptors for IgG. C3-coated red cells attached at low levels to spleen macrophages, but attachment and endocytosis were enhanced on fibronectin-coated surfaces. The ionophore A23187 induced spleen macrophages to synthesize prostaglandin E2, but like Kupffer cells, spleen macrophages did not synthesize leukotrienes and made relatively small amounts of HETE and 12-hydroxyheptadecanoic acid. Resident spleen macrophages did not produce H2O2, but splenic inflammatory cells, induced by infection of animals with Listeria monocytogenes, actively released H2O2. We conclude that the functional properties of resident, sinusoidal-lining macrophages in liver and spleen are similar to one another but distinct from other pools of phagocytes.
format Text
id pubmed-2189070
institution National Center for Biotechnology Information
language English
publishDate 1988
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-21890702008-04-17 Properties of isolated red pulp macrophages from mouse spleen J Exp Med Articles Dense monolayers of large, adherent macrophages were prepared from the red pulp of mouse spleen. These sinus-lining phagocytes resembled liver Kupffer cells in morphology, as well as expression of F4/80 and class II MHC antigens and receptors for IgG. C3-coated red cells attached at low levels to spleen macrophages, but attachment and endocytosis were enhanced on fibronectin-coated surfaces. The ionophore A23187 induced spleen macrophages to synthesize prostaglandin E2, but like Kupffer cells, spleen macrophages did not synthesize leukotrienes and made relatively small amounts of HETE and 12-hydroxyheptadecanoic acid. Resident spleen macrophages did not produce H2O2, but splenic inflammatory cells, induced by infection of animals with Listeria monocytogenes, actively released H2O2. We conclude that the functional properties of resident, sinusoidal-lining macrophages in liver and spleen are similar to one another but distinct from other pools of phagocytes. The Rockefeller University Press 1988-10-01 /pmc/articles/PMC2189070/ /pubmed/2971759 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Properties of isolated red pulp macrophages from mouse spleen
title Properties of isolated red pulp macrophages from mouse spleen
title_full Properties of isolated red pulp macrophages from mouse spleen
title_fullStr Properties of isolated red pulp macrophages from mouse spleen
title_full_unstemmed Properties of isolated red pulp macrophages from mouse spleen
title_short Properties of isolated red pulp macrophages from mouse spleen
title_sort properties of isolated red pulp macrophages from mouse spleen
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2189070/
https://www.ncbi.nlm.nih.gov/pubmed/2971759