A human urine-derived interleukin 1 inhibitor. Homology with deoxyribonuclease I

We have previously reported that the urine of febrile humans contained large quantities of an inhibitor of IL-1-induced murine thymocyte proliferation that was a glycoprotein between 30 and 40 kD in size. In the present study this factor has been purified to homogeneity using a sequence of eight pur...

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Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1988
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2189114/
https://www.ncbi.nlm.nih.gov/pubmed/3263467
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collection PubMed
description We have previously reported that the urine of febrile humans contained large quantities of an inhibitor of IL-1-induced murine thymocyte proliferation that was a glycoprotein between 30 and 40 kD in size. In the present study this factor has been purified to homogeneity using a sequence of eight purification steps (ammonium sulfate precipitation, ion exchange chromatography, molecular sieve chromatography, hydrophobic affinity chromatography, hydroxylapatite chromatography, fast protein liquid chromatography, and two HPLC steps). SDS-PAGE analysis indicates that the purified material is a 38-kD molecule. Evidence based on a partial amino acid sequence analysis as well as enzyme studies indicates that this inhibitor is a type of human DNase I.
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spelling pubmed-21891142008-04-17 A human urine-derived interleukin 1 inhibitor. Homology with deoxyribonuclease I J Exp Med Articles We have previously reported that the urine of febrile humans contained large quantities of an inhibitor of IL-1-induced murine thymocyte proliferation that was a glycoprotein between 30 and 40 kD in size. In the present study this factor has been purified to homogeneity using a sequence of eight purification steps (ammonium sulfate precipitation, ion exchange chromatography, molecular sieve chromatography, hydrophobic affinity chromatography, hydroxylapatite chromatography, fast protein liquid chromatography, and two HPLC steps). SDS-PAGE analysis indicates that the purified material is a 38-kD molecule. Evidence based on a partial amino acid sequence analysis as well as enzyme studies indicates that this inhibitor is a type of human DNase I. The Rockefeller University Press 1988-11-01 /pmc/articles/PMC2189114/ /pubmed/3263467 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
A human urine-derived interleukin 1 inhibitor. Homology with deoxyribonuclease I
title A human urine-derived interleukin 1 inhibitor. Homology with deoxyribonuclease I
title_full A human urine-derived interleukin 1 inhibitor. Homology with deoxyribonuclease I
title_fullStr A human urine-derived interleukin 1 inhibitor. Homology with deoxyribonuclease I
title_full_unstemmed A human urine-derived interleukin 1 inhibitor. Homology with deoxyribonuclease I
title_short A human urine-derived interleukin 1 inhibitor. Homology with deoxyribonuclease I
title_sort human urine-derived interleukin 1 inhibitor. homology with deoxyribonuclease i
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2189114/
https://www.ncbi.nlm.nih.gov/pubmed/3263467

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