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Alternative V kappa gene rearrangements in a murine B cell lymphoma. An explantation for idiotypic heterogeneity

Idiotype variants of 38C13, a murine B cell lymphoma, have been isolated by immunoselection with antiidiotype mAbs. The V region genes for the kappa light chains and mu heavy chains expressed by these tumor cells were sequenced and compared. There was no evidence for V region somatic point mutation...

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Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1988
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2189115/
https://www.ncbi.nlm.nih.gov/pubmed/3141553
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collection PubMed
description Idiotype variants of 38C13, a murine B cell lymphoma, have been isolated by immunoselection with antiidiotype mAbs. The V region genes for the kappa light chains and mu heavy chains expressed by these tumor cells were sequenced and compared. There was no evidence for V region somatic point mutation in this tumor. However, while the heavy chain genes were all identical, the light chain genes were all different. The light chain genes of each variant were derived from the V kappa-Ox1 gene family and joined to J kappa 4, whereas the light chain gene of the parental tumor was derived from the V kappa 9 family and joined to J kappa 2. Two of the variants used the identical V kappa gene but differed by the inclusion of a variable number of additional nucleotides in the V/J joint. Thus, the idiotypic heterogeneity of this B cell lymphoma arises as a consequence of alternative light chain rearrangements rather than point mutation. This process repetitively uses members of the same V kappa gene family. Two of the variants use the identical V kappa and J kappa gene segments but differ by the presence of extra nucleotides at the V kappa/J kappa joint.
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spelling pubmed-21891152008-04-17 Alternative V kappa gene rearrangements in a murine B cell lymphoma. An explantation for idiotypic heterogeneity J Exp Med Articles Idiotype variants of 38C13, a murine B cell lymphoma, have been isolated by immunoselection with antiidiotype mAbs. The V region genes for the kappa light chains and mu heavy chains expressed by these tumor cells were sequenced and compared. There was no evidence for V region somatic point mutation in this tumor. However, while the heavy chain genes were all identical, the light chain genes were all different. The light chain genes of each variant were derived from the V kappa-Ox1 gene family and joined to J kappa 4, whereas the light chain gene of the parental tumor was derived from the V kappa 9 family and joined to J kappa 2. Two of the variants used the identical V kappa gene but differed by the inclusion of a variable number of additional nucleotides in the V/J joint. Thus, the idiotypic heterogeneity of this B cell lymphoma arises as a consequence of alternative light chain rearrangements rather than point mutation. This process repetitively uses members of the same V kappa gene family. Two of the variants use the identical V kappa and J kappa gene segments but differ by the presence of extra nucleotides at the V kappa/J kappa joint. The Rockefeller University Press 1988-11-01 /pmc/articles/PMC2189115/ /pubmed/3141553 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Alternative V kappa gene rearrangements in a murine B cell lymphoma. An explantation for idiotypic heterogeneity
title Alternative V kappa gene rearrangements in a murine B cell lymphoma. An explantation for idiotypic heterogeneity
title_full Alternative V kappa gene rearrangements in a murine B cell lymphoma. An explantation for idiotypic heterogeneity
title_fullStr Alternative V kappa gene rearrangements in a murine B cell lymphoma. An explantation for idiotypic heterogeneity
title_full_unstemmed Alternative V kappa gene rearrangements in a murine B cell lymphoma. An explantation for idiotypic heterogeneity
title_short Alternative V kappa gene rearrangements in a murine B cell lymphoma. An explantation for idiotypic heterogeneity
title_sort alternative v kappa gene rearrangements in a murine b cell lymphoma. an explantation for idiotypic heterogeneity
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2189115/
https://www.ncbi.nlm.nih.gov/pubmed/3141553