Ordered activation of the Ig lambda locus in Abelson B cell lines

Derivatives of the mu-producing Abelson line P8 have been analyzed for L chain gene rearrangements. Two of seven clones studied assembled their V lambda genes while growing in culture. V lambda gene rearrangements occurred only in those Abelson subclones that either were rearranging or had rearrange...

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Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1988
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2189143/
https://www.ncbi.nlm.nih.gov/pubmed/2848920
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collection PubMed
description Derivatives of the mu-producing Abelson line P8 have been analyzed for L chain gene rearrangements. Two of seven clones studied assembled their V lambda genes while growing in culture. V lambda gene rearrangements occurred only in those Abelson subclones that either were rearranging or had rearranged their recombining sequence (RS) element on both Ig kappa alleles. Our data suggest that (a) RS rearrangements are preferentially initiated in kappa- pre-B cells; and (b) the deletion or inactivation of sequences lying between J kappa and RS is a requirement for the activation of the Ig lambda locus.
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spelling pubmed-21891432008-04-17 Ordered activation of the Ig lambda locus in Abelson B cell lines J Exp Med Articles Derivatives of the mu-producing Abelson line P8 have been analyzed for L chain gene rearrangements. Two of seven clones studied assembled their V lambda genes while growing in culture. V lambda gene rearrangements occurred only in those Abelson subclones that either were rearranging or had rearranged their recombining sequence (RS) element on both Ig kappa alleles. Our data suggest that (a) RS rearrangements are preferentially initiated in kappa- pre-B cells; and (b) the deletion or inactivation of sequences lying between J kappa and RS is a requirement for the activation of the Ig lambda locus. The Rockefeller University Press 1988-12-01 /pmc/articles/PMC2189143/ /pubmed/2848920 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Ordered activation of the Ig lambda locus in Abelson B cell lines
title Ordered activation of the Ig lambda locus in Abelson B cell lines
title_full Ordered activation of the Ig lambda locus in Abelson B cell lines
title_fullStr Ordered activation of the Ig lambda locus in Abelson B cell lines
title_full_unstemmed Ordered activation of the Ig lambda locus in Abelson B cell lines
title_short Ordered activation of the Ig lambda locus in Abelson B cell lines
title_sort ordered activation of the ig lambda locus in abelson b cell lines
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2189143/
https://www.ncbi.nlm.nih.gov/pubmed/2848920

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