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Interferon gamma and lipopolysaccharide promote macrophage adherence to basement membrane glycoproteins

The ability of thioglycollate (TG)-elicited peritoneal macrophages, a population of recently recruited monocytes, to adhere to the basement membrane glycoproteins laminin and type IV collagen is not a constitutive function of these cells. Adherence can be induced, however, by treatment with IFN-gamm...

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Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1989
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2189188/
https://www.ncbi.nlm.nih.gov/pubmed/2491881
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description The ability of thioglycollate (TG)-elicited peritoneal macrophages, a population of recently recruited monocytes, to adhere to the basement membrane glycoproteins laminin and type IV collagen is not a constitutive function of these cells. Adherence can be induced, however, by treatment with IFN-gamma and LPS. In general, IFN-gamma is more potent than LPS in promoting this adherence. Maximal adherence, however, is observed when IFN-gamma (greater than or equal to 5 U/ml) is used together with LPS (2.0 ng/ml). These requirements parallel the conditions needed to obtain tumoricidal activation of TG-elicited macrophages. Adherence to laminin, in the presence of these stimuli, is transient, being maximal at 8 h after their addition and diminishing with longer periods of incubation. In contrast, adherence to type IV collagen does not appear to be transient and IFN-gamma and LPS induce a more prolonged association of macrophages with this substratum.
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spelling pubmed-21891882008-04-17 Interferon gamma and lipopolysaccharide promote macrophage adherence to basement membrane glycoproteins J Exp Med Articles The ability of thioglycollate (TG)-elicited peritoneal macrophages, a population of recently recruited monocytes, to adhere to the basement membrane glycoproteins laminin and type IV collagen is not a constitutive function of these cells. Adherence can be induced, however, by treatment with IFN-gamma and LPS. In general, IFN-gamma is more potent than LPS in promoting this adherence. Maximal adherence, however, is observed when IFN-gamma (greater than or equal to 5 U/ml) is used together with LPS (2.0 ng/ml). These requirements parallel the conditions needed to obtain tumoricidal activation of TG-elicited macrophages. Adherence to laminin, in the presence of these stimuli, is transient, being maximal at 8 h after their addition and diminishing with longer periods of incubation. In contrast, adherence to type IV collagen does not appear to be transient and IFN-gamma and LPS induce a more prolonged association of macrophages with this substratum. The Rockefeller University Press 1989-01-01 /pmc/articles/PMC2189188/ /pubmed/2491881 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Interferon gamma and lipopolysaccharide promote macrophage adherence to basement membrane glycoproteins
title Interferon gamma and lipopolysaccharide promote macrophage adherence to basement membrane glycoproteins
title_full Interferon gamma and lipopolysaccharide promote macrophage adherence to basement membrane glycoproteins
title_fullStr Interferon gamma and lipopolysaccharide promote macrophage adherence to basement membrane glycoproteins
title_full_unstemmed Interferon gamma and lipopolysaccharide promote macrophage adherence to basement membrane glycoproteins
title_short Interferon gamma and lipopolysaccharide promote macrophage adherence to basement membrane glycoproteins
title_sort interferon gamma and lipopolysaccharide promote macrophage adherence to basement membrane glycoproteins
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2189188/
https://www.ncbi.nlm.nih.gov/pubmed/2491881