Recognition of oligonucleotide-encoded T cell epitopes introduced into a gene unrelated to the original antigen

We have previously demonstrated that H-2Kd-restricted CTL specific for HLA-CW3 or HLA-A24 can recognize synthetic peptides corresponding to residues 170-182 of the HLA molecules. Synthetic oligonucleotides encoding region 170-182 of CW3 or A24 were inserted into the influenza nucleoprotein (NP) gene...

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Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1989
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2189194/
https://www.ncbi.nlm.nih.gov/pubmed/2462610
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description We have previously demonstrated that H-2Kd-restricted CTL specific for HLA-CW3 or HLA-A24 can recognize synthetic peptides corresponding to residues 170-182 of the HLA molecules. Synthetic oligonucleotides encoding region 170-182 of CW3 or A24 were inserted into the influenza nucleoprotein (NP) gene. We demonstrate herein that P815 (H-2d) cells transfected with the NP-oligo recombinant genes are specifically lysed by HLA-specific Kd-restricted CTL clones. Our results imply that there must be a high degree of flexibility for the expression of T cell epitopes in different molecular contexts.
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spelling pubmed-21891942008-04-17 Recognition of oligonucleotide-encoded T cell epitopes introduced into a gene unrelated to the original antigen J Exp Med Articles We have previously demonstrated that H-2Kd-restricted CTL specific for HLA-CW3 or HLA-A24 can recognize synthetic peptides corresponding to residues 170-182 of the HLA molecules. Synthetic oligonucleotides encoding region 170-182 of CW3 or A24 were inserted into the influenza nucleoprotein (NP) gene. We demonstrate herein that P815 (H-2d) cells transfected with the NP-oligo recombinant genes are specifically lysed by HLA-specific Kd-restricted CTL clones. Our results imply that there must be a high degree of flexibility for the expression of T cell epitopes in different molecular contexts. The Rockefeller University Press 1989-01-01 /pmc/articles/PMC2189194/ /pubmed/2462610 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Recognition of oligonucleotide-encoded T cell epitopes introduced into a gene unrelated to the original antigen
title Recognition of oligonucleotide-encoded T cell epitopes introduced into a gene unrelated to the original antigen
title_full Recognition of oligonucleotide-encoded T cell epitopes introduced into a gene unrelated to the original antigen
title_fullStr Recognition of oligonucleotide-encoded T cell epitopes introduced into a gene unrelated to the original antigen
title_full_unstemmed Recognition of oligonucleotide-encoded T cell epitopes introduced into a gene unrelated to the original antigen
title_short Recognition of oligonucleotide-encoded T cell epitopes introduced into a gene unrelated to the original antigen
title_sort recognition of oligonucleotide-encoded t cell epitopes introduced into a gene unrelated to the original antigen
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2189194/
https://www.ncbi.nlm.nih.gov/pubmed/2462610

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