Purification and characterization of a novel monocyte chemotactic and activating factor produced by a human myelomonocytic cell line

A novel basic heparin-binding monocyte chemotactic factor (MCF) was purified to homogeneity from the conditioned media of human myelomonocytic cell line THP-1 based on its in vitro monocyte chemotactic activity. The purified MCF was homogenous and estimated to be 15 kD on SDS-PAGE. Purified MCF stim...

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Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1989
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2189236/
https://www.ncbi.nlm.nih.gov/pubmed/2926331
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description A novel basic heparin-binding monocyte chemotactic factor (MCF) was purified to homogeneity from the conditioned media of human myelomonocytic cell line THP-1 based on its in vitro monocyte chemotactic activity. The purified MCF was homogenous and estimated to be 15 kD on SDS-PAGE. Purified MCF stimulated normal human monocytes to be growth inhibitory in vitro at 2-3 d for several human tumor cell lines. This represents the first report of the identification and purification of a chemoattractant cytokine that also activates monocytes but is distinct from interferons and other known cytokines.
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spelling pubmed-21892362008-04-17 Purification and characterization of a novel monocyte chemotactic and activating factor produced by a human myelomonocytic cell line J Exp Med Articles A novel basic heparin-binding monocyte chemotactic factor (MCF) was purified to homogeneity from the conditioned media of human myelomonocytic cell line THP-1 based on its in vitro monocyte chemotactic activity. The purified MCF was homogenous and estimated to be 15 kD on SDS-PAGE. Purified MCF stimulated normal human monocytes to be growth inhibitory in vitro at 2-3 d for several human tumor cell lines. This represents the first report of the identification and purification of a chemoattractant cytokine that also activates monocytes but is distinct from interferons and other known cytokines. The Rockefeller University Press 1989-04-01 /pmc/articles/PMC2189236/ /pubmed/2926331 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Purification and characterization of a novel monocyte chemotactic and activating factor produced by a human myelomonocytic cell line
title Purification and characterization of a novel monocyte chemotactic and activating factor produced by a human myelomonocytic cell line
title_full Purification and characterization of a novel monocyte chemotactic and activating factor produced by a human myelomonocytic cell line
title_fullStr Purification and characterization of a novel monocyte chemotactic and activating factor produced by a human myelomonocytic cell line
title_full_unstemmed Purification and characterization of a novel monocyte chemotactic and activating factor produced by a human myelomonocytic cell line
title_short Purification and characterization of a novel monocyte chemotactic and activating factor produced by a human myelomonocytic cell line
title_sort purification and characterization of a novel monocyte chemotactic and activating factor produced by a human myelomonocytic cell line
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2189236/
https://www.ncbi.nlm.nih.gov/pubmed/2926331

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