Role of superoxide anion radicals in ethanol metabolism by blood monocyte-derived human macrophages

The effects of a number of additives on the rate of conversion of ethanol (1 mg/ml; 21.7 mM) to acetate by monolayers of blood monocyte- derived human macrophages were investigated. The additives studied were superoxide dismutase (SOD; 1,500 U/ml), catalase (1,500 U/ml), tetrahydrofurane (20 mM), an...

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Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1989
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Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2189258/
https://www.ncbi.nlm.nih.gov/pubmed/2538545
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description The effects of a number of additives on the rate of conversion of ethanol (1 mg/ml; 21.7 mM) to acetate by monolayers of blood monocyte- derived human macrophages were investigated. The additives studied were superoxide dismutase (SOD; 1,500 U/ml), catalase (1,500 U/ml), tetrahydrofurane (20 mM), and PMA (20 nM), either singly or in various combinations. SOD, catalase, SOD plus catalase, tetrahydrofurane, and tetrahydrofurane plus SOD inhibited ethanol oxidation by 49.2, 12.1, 52.9, 60.4, and 66.8%, respectively. PMA caused a 4.0-8.3-fold increase in the rate of ethanol metabolism and this increase was completely suppressed in the presence of SOD. The data indicate that a substantial proportion of the ethanol metabolism by both unstimulated and PMA- stimulated blood monocyte-derived macrophages was dependent on the generation of superoxide anion radicals.
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spelling pubmed-21892582008-04-17 Role of superoxide anion radicals in ethanol metabolism by blood monocyte-derived human macrophages J Exp Med Articles The effects of a number of additives on the rate of conversion of ethanol (1 mg/ml; 21.7 mM) to acetate by monolayers of blood monocyte- derived human macrophages were investigated. The additives studied were superoxide dismutase (SOD; 1,500 U/ml), catalase (1,500 U/ml), tetrahydrofurane (20 mM), and PMA (20 nM), either singly or in various combinations. SOD, catalase, SOD plus catalase, tetrahydrofurane, and tetrahydrofurane plus SOD inhibited ethanol oxidation by 49.2, 12.1, 52.9, 60.4, and 66.8%, respectively. PMA caused a 4.0-8.3-fold increase in the rate of ethanol metabolism and this increase was completely suppressed in the presence of SOD. The data indicate that a substantial proportion of the ethanol metabolism by both unstimulated and PMA- stimulated blood monocyte-derived macrophages was dependent on the generation of superoxide anion radicals. The Rockefeller University Press 1989-03-01 /pmc/articles/PMC2189258/ /pubmed/2538545 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Role of superoxide anion radicals in ethanol metabolism by blood monocyte-derived human macrophages
title Role of superoxide anion radicals in ethanol metabolism by blood monocyte-derived human macrophages
title_full Role of superoxide anion radicals in ethanol metabolism by blood monocyte-derived human macrophages
title_fullStr Role of superoxide anion radicals in ethanol metabolism by blood monocyte-derived human macrophages
title_full_unstemmed Role of superoxide anion radicals in ethanol metabolism by blood monocyte-derived human macrophages
title_short Role of superoxide anion radicals in ethanol metabolism by blood monocyte-derived human macrophages
title_sort role of superoxide anion radicals in ethanol metabolism by blood monocyte-derived human macrophages
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2189258/
https://www.ncbi.nlm.nih.gov/pubmed/2538545

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