Interferon alpha associated with systemic lupus erythematosus is not intrinsically acid labile

The physicochemical properties of apparently acid-labile IFN-alpha from patients with SLE have been studied. The antigenicity, apparent molecular size, and isoelectric point of SLE IFN-alpha are indistinguishable from those of conventional, previously characterized, acid-stable subspecies of IFN-alp...

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Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1989
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2189267/
https://www.ncbi.nlm.nih.gov/pubmed/2926326
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description The physicochemical properties of apparently acid-labile IFN-alpha from patients with SLE have been studied. The antigenicity, apparent molecular size, and isoelectric point of SLE IFN-alpha are indistinguishable from those of conventional, previously characterized, acid-stable subspecies of IFN-alpha. However, after partial purification by anion-exchange chromatography, SLE IFN-alpha no longer exhibits acid lability, suggesting that other plasma factor(s) are responsible for the acid lability of SLE IFN-alpha. Addition of SLE plasma, but not normal plasma, to conventional acid-stable IFN-alpha renders the exogenous IFN-alpha acid labile. Preliminary results demonstrate that an acid-dependent IFN-inactivating activity can be partially purified from SLE plasma by anion-exchange chromatography.
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spelling pubmed-21892672008-04-17 Interferon alpha associated with systemic lupus erythematosus is not intrinsically acid labile J Exp Med Articles The physicochemical properties of apparently acid-labile IFN-alpha from patients with SLE have been studied. The antigenicity, apparent molecular size, and isoelectric point of SLE IFN-alpha are indistinguishable from those of conventional, previously characterized, acid-stable subspecies of IFN-alpha. However, after partial purification by anion-exchange chromatography, SLE IFN-alpha no longer exhibits acid lability, suggesting that other plasma factor(s) are responsible for the acid lability of SLE IFN-alpha. Addition of SLE plasma, but not normal plasma, to conventional acid-stable IFN-alpha renders the exogenous IFN-alpha acid labile. Preliminary results demonstrate that an acid-dependent IFN-inactivating activity can be partially purified from SLE plasma by anion-exchange chromatography. The Rockefeller University Press 1989-03-01 /pmc/articles/PMC2189267/ /pubmed/2926326 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Interferon alpha associated with systemic lupus erythematosus is not intrinsically acid labile
title Interferon alpha associated with systemic lupus erythematosus is not intrinsically acid labile
title_full Interferon alpha associated with systemic lupus erythematosus is not intrinsically acid labile
title_fullStr Interferon alpha associated with systemic lupus erythematosus is not intrinsically acid labile
title_full_unstemmed Interferon alpha associated with systemic lupus erythematosus is not intrinsically acid labile
title_short Interferon alpha associated with systemic lupus erythematosus is not intrinsically acid labile
title_sort interferon alpha associated with systemic lupus erythematosus is not intrinsically acid labile
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2189267/
https://www.ncbi.nlm.nih.gov/pubmed/2926326

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