Genetic analysis of the human tumor necrosis factor alpha/cachectin promoter region in a macrophage cell line

The 615-bp 5' flanking region of the human TNF-alpha/cachectin gene was isolated and ligated to the luciferase reporter gene. In addition, a series of truncated promoter constructs was generated by exonuclease III digestion. The promoter activity of these constructs was studied in a transient t...

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Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1989
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2189360/
https://www.ncbi.nlm.nih.gov/pubmed/2746161
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collection PubMed
description The 615-bp 5' flanking region of the human TNF-alpha/cachectin gene was isolated and ligated to the luciferase reporter gene. In addition, a series of truncated promoter constructs was generated by exonuclease III digestion. The promoter activity of these constructs was studied in a transient transfection system using the TNF-alpha-producing U937 cell line. Full-length and truncated TNF promoter constructions extending from -615 to -95 bp relative to the transcription start site (TSS) could be induced by phorbol esters. A construct truncated to within 36 bp of the TSS (and within 11 bp of the TATAA box) was inactive. Therefore, the phorbol ester responsive is localized in the TNF/cachectin promoter to a relatively short region proximal to the TATAA box.
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spelling pubmed-21893602008-04-17 Genetic analysis of the human tumor necrosis factor alpha/cachectin promoter region in a macrophage cell line J Exp Med Articles The 615-bp 5' flanking region of the human TNF-alpha/cachectin gene was isolated and ligated to the luciferase reporter gene. In addition, a series of truncated promoter constructs was generated by exonuclease III digestion. The promoter activity of these constructs was studied in a transient transfection system using the TNF-alpha-producing U937 cell line. Full-length and truncated TNF promoter constructions extending from -615 to -95 bp relative to the transcription start site (TSS) could be induced by phorbol esters. A construct truncated to within 36 bp of the TSS (and within 11 bp of the TATAA box) was inactive. Therefore, the phorbol ester responsive is localized in the TNF/cachectin promoter to a relatively short region proximal to the TATAA box. The Rockefeller University Press 1989-07-01 /pmc/articles/PMC2189360/ /pubmed/2746161 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Genetic analysis of the human tumor necrosis factor alpha/cachectin promoter region in a macrophage cell line
title Genetic analysis of the human tumor necrosis factor alpha/cachectin promoter region in a macrophage cell line
title_full Genetic analysis of the human tumor necrosis factor alpha/cachectin promoter region in a macrophage cell line
title_fullStr Genetic analysis of the human tumor necrosis factor alpha/cachectin promoter region in a macrophage cell line
title_full_unstemmed Genetic analysis of the human tumor necrosis factor alpha/cachectin promoter region in a macrophage cell line
title_short Genetic analysis of the human tumor necrosis factor alpha/cachectin promoter region in a macrophage cell line
title_sort genetic analysis of the human tumor necrosis factor alpha/cachectin promoter region in a macrophage cell line
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2189360/
https://www.ncbi.nlm.nih.gov/pubmed/2746161

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