Establishment of human double-positive thymocyte clones

Human thymocytes were sorted according to the expression of CD4 and CD8 molecules and clones representing the four subpopulations (DP, DN, and single CD4 or CD8 positive) were established. DP clones can be maintained for long periods in tissue culture and give rise to a variable percentage of SP var...

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Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1989
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2189367/
https://www.ncbi.nlm.nih.gov/pubmed/2501447
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collection PubMed
description Human thymocytes were sorted according to the expression of CD4 and CD8 molecules and clones representing the four subpopulations (DP, DN, and single CD4 or CD8 positive) were established. DP clones can be maintained for long periods in tissue culture and give rise to a variable percentage of SP variants. These variants, when isolated and further expanded, do not revert to a DP phenotype. DP clones express a functional TCR-CD3 complex, suggesting that this molecule can interact with the thymic microenvironment during T cell differentiation.
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spelling pubmed-21893672008-04-17 Establishment of human double-positive thymocyte clones J Exp Med Articles Human thymocytes were sorted according to the expression of CD4 and CD8 molecules and clones representing the four subpopulations (DP, DN, and single CD4 or CD8 positive) were established. DP clones can be maintained for long periods in tissue culture and give rise to a variable percentage of SP variants. These variants, when isolated and further expanded, do not revert to a DP phenotype. DP clones express a functional TCR-CD3 complex, suggesting that this molecule can interact with the thymic microenvironment during T cell differentiation. The Rockefeller University Press 1989-07-01 /pmc/articles/PMC2189367/ /pubmed/2501447 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Establishment of human double-positive thymocyte clones
title Establishment of human double-positive thymocyte clones
title_full Establishment of human double-positive thymocyte clones
title_fullStr Establishment of human double-positive thymocyte clones
title_full_unstemmed Establishment of human double-positive thymocyte clones
title_short Establishment of human double-positive thymocyte clones
title_sort establishment of human double-positive thymocyte clones
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2189367/
https://www.ncbi.nlm.nih.gov/pubmed/2501447

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