Cargando…
Alveolar macrophage elimination in vivo is associated with an increase in pulmonary immune response in mice
A single intracheal dose of liposome-encapsuled dichloro-methylene- diphosphonate resulted in the elimination of alveolar macrophages (AM) from the lung, creating a model to study the in vivo role of AM in the pulmonary immune response. Using intratracheally administered trinitrophenyl-keyhole limpe...
Formato: | Texto |
---|---|
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
1989
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2189410/ https://www.ncbi.nlm.nih.gov/pubmed/2526847 |
_version_ | 1782146635756732416 |
---|---|
collection | PubMed |
description | A single intracheal dose of liposome-encapsuled dichloro-methylene- diphosphonate resulted in the elimination of alveolar macrophages (AM) from the lung, creating a model to study the in vivo role of AM in the pulmonary immune response. Using intratracheally administered trinitrophenyl-keyhole limpet hemocyanin (TNP-KLH), the kinetics of the response, the location and number of TNP-specific antibody-forming cells, and the different Ig classes of the antibodies produced were studied in AM-depleted animals. The results show that AM elimination has a dramatic effect on the pulmonary immune responses against TNP- KLH. An increase in APC in lung-associated lymph nodes and a prolongation of the response is found, as well as an introduction of APC in lung tissue. In both experimental groups, the majority of the TNP-specific antibodies produced was IgG, followed by IgA and IgE, while very few IgM antibodies could be detected. We conclude from these results that AM are likely to play a role in controlling the pulmonary immune response in a suppressive way, thereby limiting the possible damage caused by severe immune responses in lung tissue. |
format | Text |
id | pubmed-2189410 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1989 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21894102008-04-17 Alveolar macrophage elimination in vivo is associated with an increase in pulmonary immune response in mice J Exp Med Articles A single intracheal dose of liposome-encapsuled dichloro-methylene- diphosphonate resulted in the elimination of alveolar macrophages (AM) from the lung, creating a model to study the in vivo role of AM in the pulmonary immune response. Using intratracheally administered trinitrophenyl-keyhole limpet hemocyanin (TNP-KLH), the kinetics of the response, the location and number of TNP-specific antibody-forming cells, and the different Ig classes of the antibodies produced were studied in AM-depleted animals. The results show that AM elimination has a dramatic effect on the pulmonary immune responses against TNP- KLH. An increase in APC in lung-associated lymph nodes and a prolongation of the response is found, as well as an introduction of APC in lung tissue. In both experimental groups, the majority of the TNP-specific antibodies produced was IgG, followed by IgA and IgE, while very few IgM antibodies could be detected. We conclude from these results that AM are likely to play a role in controlling the pulmonary immune response in a suppressive way, thereby limiting the possible damage caused by severe immune responses in lung tissue. The Rockefeller University Press 1989-08-01 /pmc/articles/PMC2189410/ /pubmed/2526847 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Articles Alveolar macrophage elimination in vivo is associated with an increase in pulmonary immune response in mice |
title | Alveolar macrophage elimination in vivo is associated with an increase in pulmonary immune response in mice |
title_full | Alveolar macrophage elimination in vivo is associated with an increase in pulmonary immune response in mice |
title_fullStr | Alveolar macrophage elimination in vivo is associated with an increase in pulmonary immune response in mice |
title_full_unstemmed | Alveolar macrophage elimination in vivo is associated with an increase in pulmonary immune response in mice |
title_short | Alveolar macrophage elimination in vivo is associated with an increase in pulmonary immune response in mice |
title_sort | alveolar macrophage elimination in vivo is associated with an increase in pulmonary immune response in mice |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2189410/ https://www.ncbi.nlm.nih.gov/pubmed/2526847 |