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Alveolar macrophage elimination in vivo is associated with an increase in pulmonary immune response in mice

A single intracheal dose of liposome-encapsuled dichloro-methylene- diphosphonate resulted in the elimination of alveolar macrophages (AM) from the lung, creating a model to study the in vivo role of AM in the pulmonary immune response. Using intratracheally administered trinitrophenyl-keyhole limpe...

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Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1989
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2189410/
https://www.ncbi.nlm.nih.gov/pubmed/2526847
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description A single intracheal dose of liposome-encapsuled dichloro-methylene- diphosphonate resulted in the elimination of alveolar macrophages (AM) from the lung, creating a model to study the in vivo role of AM in the pulmonary immune response. Using intratracheally administered trinitrophenyl-keyhole limpet hemocyanin (TNP-KLH), the kinetics of the response, the location and number of TNP-specific antibody-forming cells, and the different Ig classes of the antibodies produced were studied in AM-depleted animals. The results show that AM elimination has a dramatic effect on the pulmonary immune responses against TNP- KLH. An increase in APC in lung-associated lymph nodes and a prolongation of the response is found, as well as an introduction of APC in lung tissue. In both experimental groups, the majority of the TNP-specific antibodies produced was IgG, followed by IgA and IgE, while very few IgM antibodies could be detected. We conclude from these results that AM are likely to play a role in controlling the pulmonary immune response in a suppressive way, thereby limiting the possible damage caused by severe immune responses in lung tissue.
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spelling pubmed-21894102008-04-17 Alveolar macrophage elimination in vivo is associated with an increase in pulmonary immune response in mice J Exp Med Articles A single intracheal dose of liposome-encapsuled dichloro-methylene- diphosphonate resulted in the elimination of alveolar macrophages (AM) from the lung, creating a model to study the in vivo role of AM in the pulmonary immune response. Using intratracheally administered trinitrophenyl-keyhole limpet hemocyanin (TNP-KLH), the kinetics of the response, the location and number of TNP-specific antibody-forming cells, and the different Ig classes of the antibodies produced were studied in AM-depleted animals. The results show that AM elimination has a dramatic effect on the pulmonary immune responses against TNP- KLH. An increase in APC in lung-associated lymph nodes and a prolongation of the response is found, as well as an introduction of APC in lung tissue. In both experimental groups, the majority of the TNP-specific antibodies produced was IgG, followed by IgA and IgE, while very few IgM antibodies could be detected. We conclude from these results that AM are likely to play a role in controlling the pulmonary immune response in a suppressive way, thereby limiting the possible damage caused by severe immune responses in lung tissue. The Rockefeller University Press 1989-08-01 /pmc/articles/PMC2189410/ /pubmed/2526847 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Alveolar macrophage elimination in vivo is associated with an increase in pulmonary immune response in mice
title Alveolar macrophage elimination in vivo is associated with an increase in pulmonary immune response in mice
title_full Alveolar macrophage elimination in vivo is associated with an increase in pulmonary immune response in mice
title_fullStr Alveolar macrophage elimination in vivo is associated with an increase in pulmonary immune response in mice
title_full_unstemmed Alveolar macrophage elimination in vivo is associated with an increase in pulmonary immune response in mice
title_short Alveolar macrophage elimination in vivo is associated with an increase in pulmonary immune response in mice
title_sort alveolar macrophage elimination in vivo is associated with an increase in pulmonary immune response in mice
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2189410/
https://www.ncbi.nlm.nih.gov/pubmed/2526847