Cloning and sequencing of a cDNA expressing a recombinant house dust mite protein that binds human IgE and corresponds to an important low molecular weight allergen [published erratum appears in J Exp Med 1990 Apr 1;171(4):1387]

A cDNA clone coding for a mite allergen of mol wt approximately 14,000 has been isolated and its DNA sequence determined. The native component from mite extracts encoded by this DNA was identified by immunoprobing blots of mite body extract with human IgE eluted from the electroblotted cloned fusion...

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Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1989
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Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2189463/
https://www.ncbi.nlm.nih.gov/pubmed/2794865
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collection PubMed
description A cDNA clone coding for a mite allergen of mol wt approximately 14,000 has been isolated and its DNA sequence determined. The native component from mite extracts encoded by this DNA was identified by immunoprobing blots of mite body extract with human IgE eluted from the electroblotted cloned fusion polypeptides derived from the expressed cDNA clone. The clone encodes a polypeptide with a deduced mol wt of 17,460. The deduced amino acid sequence was not homologous to any known protein sequences and it contains no cysteine or tryptophan. On blots, 21 of 38 sera from mite-allergic subjects recognized the cloned material, and this recognition strongly correlated with IgE-binding to the native component on protein blots of mite extract.
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spelling pubmed-21894632008-04-17 Cloning and sequencing of a cDNA expressing a recombinant house dust mite protein that binds human IgE and corresponds to an important low molecular weight allergen [published erratum appears in J Exp Med 1990 Apr 1;171(4):1387] J Exp Med Articles A cDNA clone coding for a mite allergen of mol wt approximately 14,000 has been isolated and its DNA sequence determined. The native component from mite extracts encoded by this DNA was identified by immunoprobing blots of mite body extract with human IgE eluted from the electroblotted cloned fusion polypeptides derived from the expressed cDNA clone. The clone encodes a polypeptide with a deduced mol wt of 17,460. The deduced amino acid sequence was not homologous to any known protein sequences and it contains no cysteine or tryptophan. On blots, 21 of 38 sera from mite-allergic subjects recognized the cloned material, and this recognition strongly correlated with IgE-binding to the native component on protein blots of mite extract. The Rockefeller University Press 1989-10-01 /pmc/articles/PMC2189463/ /pubmed/2794865 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Cloning and sequencing of a cDNA expressing a recombinant house dust mite protein that binds human IgE and corresponds to an important low molecular weight allergen [published erratum appears in J Exp Med 1990 Apr 1;171(4):1387]
title Cloning and sequencing of a cDNA expressing a recombinant house dust mite protein that binds human IgE and corresponds to an important low molecular weight allergen [published erratum appears in J Exp Med 1990 Apr 1;171(4):1387]
title_full Cloning and sequencing of a cDNA expressing a recombinant house dust mite protein that binds human IgE and corresponds to an important low molecular weight allergen [published erratum appears in J Exp Med 1990 Apr 1;171(4):1387]
title_fullStr Cloning and sequencing of a cDNA expressing a recombinant house dust mite protein that binds human IgE and corresponds to an important low molecular weight allergen [published erratum appears in J Exp Med 1990 Apr 1;171(4):1387]
title_full_unstemmed Cloning and sequencing of a cDNA expressing a recombinant house dust mite protein that binds human IgE and corresponds to an important low molecular weight allergen [published erratum appears in J Exp Med 1990 Apr 1;171(4):1387]
title_short Cloning and sequencing of a cDNA expressing a recombinant house dust mite protein that binds human IgE and corresponds to an important low molecular weight allergen [published erratum appears in J Exp Med 1990 Apr 1;171(4):1387]
title_sort cloning and sequencing of a cdna expressing a recombinant house dust mite protein that binds human ige and corresponds to an important low molecular weight allergen [published erratum appears in j exp med 1990 apr 1;171(4):1387]
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2189463/
https://www.ncbi.nlm.nih.gov/pubmed/2794865

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