Identification of a functional NF-kappa B binding site in the murine T cell receptor beta 2 locus

We have identified a sequence in the TCR beta 2 locus that is homologous to the kappa B site in the Ig kappa light chain enhancer. This element, TCR beta-B, is located in the vicinity of previously identified T cell-specific DNase1 hypersensitive sites. Transfection analysis shows that a 60-bp fragm...

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Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1989
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2189503/
https://www.ncbi.nlm.nih.gov/pubmed/2530301
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collection PubMed
description We have identified a sequence in the TCR beta 2 locus that is homologous to the kappa B site in the Ig kappa light chain enhancer. This element, TCR beta-B, is located in the vicinity of previously identified T cell-specific DNase1 hypersensitive sites. Transfection analysis shows that a 60-bp fragment encompassing this site is preferentially active in T cells stimulated with phorbol esters or the HTLV-1 tax gene product compared with a B cell line that constitutively expresses NF-kappa B. Our results provide the first evidence for transcriptional regulatory sequences residing within the J beta 2-C beta 2 intron and suggest the possible involvement of these sequences in modulation of TCR beta gene expression upon cellular activation.
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spelling pubmed-21895032008-04-17 Identification of a functional NF-kappa B binding site in the murine T cell receptor beta 2 locus J Exp Med Articles We have identified a sequence in the TCR beta 2 locus that is homologous to the kappa B site in the Ig kappa light chain enhancer. This element, TCR beta-B, is located in the vicinity of previously identified T cell-specific DNase1 hypersensitive sites. Transfection analysis shows that a 60-bp fragment encompassing this site is preferentially active in T cells stimulated with phorbol esters or the HTLV-1 tax gene product compared with a B cell line that constitutively expresses NF-kappa B. Our results provide the first evidence for transcriptional regulatory sequences residing within the J beta 2-C beta 2 intron and suggest the possible involvement of these sequences in modulation of TCR beta gene expression upon cellular activation. The Rockefeller University Press 1989-11-01 /pmc/articles/PMC2189503/ /pubmed/2530301 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Identification of a functional NF-kappa B binding site in the murine T cell receptor beta 2 locus
title Identification of a functional NF-kappa B binding site in the murine T cell receptor beta 2 locus
title_full Identification of a functional NF-kappa B binding site in the murine T cell receptor beta 2 locus
title_fullStr Identification of a functional NF-kappa B binding site in the murine T cell receptor beta 2 locus
title_full_unstemmed Identification of a functional NF-kappa B binding site in the murine T cell receptor beta 2 locus
title_short Identification of a functional NF-kappa B binding site in the murine T cell receptor beta 2 locus
title_sort identification of a functional nf-kappa b binding site in the murine t cell receptor beta 2 locus
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2189503/
https://www.ncbi.nlm.nih.gov/pubmed/2530301

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