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Separation of antigen-specific lymphocytes. I. Enrichment of antigen- binding cells
Normal mouse spleen cells were fractionated in dishes coated with thin layers of DNP-gelatin or NIP-gelatin, which were insoluble at 4 degrees C. Highly viable cells were recovered from the dishes by melting the gel at 37 degrees C. NIP3- gelatin layers bound approximately 0.1% and DNP4-gelatin laye...
| Formato: | Texto |
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| Lenguaje: | English |
| Publicado: |
The Rockefeller University Press
1975
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2189776/ https://www.ncbi.nlm.nih.gov/pubmed/47891 |