Studies on the selective lysis and purification of Trypanosoma cruzi

The mechanism by which culture forms of Trypanosoma cruzi are lysed by normal mammalian sera was examined. Lysis is restricted to the epimastigote form of the organism and is not dependent on the presence of agglutinins. Lysis is a complement-dependent process, the activity being generated by the al...

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Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1975
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2189871/
https://www.ncbi.nlm.nih.gov/pubmed/807672
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description The mechanism by which culture forms of Trypanosoma cruzi are lysed by normal mammalian sera was examined. Lysis is restricted to the epimastigote form of the organism and is not dependent on the presence of agglutinins. Lysis is a complement-dependent process, the activity being generated by the alternate pathway. The selective lysis by serum was exploited to purify viable trypomastigotes by means of centrifugation in an albumin column. Essentially pure trypomastigote populations are now being employed in cell culture experiments.
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spelling pubmed-21898712008-04-17 Studies on the selective lysis and purification of Trypanosoma cruzi J Exp Med Articles The mechanism by which culture forms of Trypanosoma cruzi are lysed by normal mammalian sera was examined. Lysis is restricted to the epimastigote form of the organism and is not dependent on the presence of agglutinins. Lysis is a complement-dependent process, the activity being generated by the alternate pathway. The selective lysis by serum was exploited to purify viable trypomastigotes by means of centrifugation in an albumin column. Essentially pure trypomastigote populations are now being employed in cell culture experiments. The Rockefeller University Press 1975-07-01 /pmc/articles/PMC2189871/ /pubmed/807672 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Studies on the selective lysis and purification of Trypanosoma cruzi
title Studies on the selective lysis and purification of Trypanosoma cruzi
title_full Studies on the selective lysis and purification of Trypanosoma cruzi
title_fullStr Studies on the selective lysis and purification of Trypanosoma cruzi
title_full_unstemmed Studies on the selective lysis and purification of Trypanosoma cruzi
title_short Studies on the selective lysis and purification of Trypanosoma cruzi
title_sort studies on the selective lysis and purification of trypanosoma cruzi
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2189871/
https://www.ncbi.nlm.nih.gov/pubmed/807672

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