Clonal growth of hamster free alveolar cells in soft agar

Free alveolar cells obtained from healthy unstimulated hamsters were tested for their ability to form colonies in soft agar. Every bronchial washing so far tested contained colon-forming cells. The average plating efficiency was 8.1% (2.4-18.3%). Alveolar colony-forming cells were characterized by h...

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Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1975
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2189945/
https://www.ncbi.nlm.nih.gov/pubmed/1080792
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collection PubMed
description Free alveolar cells obtained from healthy unstimulated hamsters were tested for their ability to form colonies in soft agar. Every bronchial washing so far tested contained colon-forming cells. The average plating efficiency was 8.1% (2.4-18.3%). Alveolar colony-forming cells were characterized by having a long initial lag period (4-8 days) and only mononuclear phagocytes were found in the colony. Medium conditioned by baby hamster kidney cells or other cells was required for the initiation and maintenance of their growth. Alveolar cells from normal mice and rats also formed colonies under appropriate culture conditions.
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spelling pubmed-21899452008-04-17 Clonal growth of hamster free alveolar cells in soft agar J Exp Med Articles Free alveolar cells obtained from healthy unstimulated hamsters were tested for their ability to form colonies in soft agar. Every bronchial washing so far tested contained colon-forming cells. The average plating efficiency was 8.1% (2.4-18.3%). Alveolar colony-forming cells were characterized by having a long initial lag period (4-8 days) and only mononuclear phagocytes were found in the colony. Medium conditioned by baby hamster kidney cells or other cells was required for the initiation and maintenance of their growth. Alveolar cells from normal mice and rats also formed colonies under appropriate culture conditions. The Rockefeller University Press 1975-10-01 /pmc/articles/PMC2189945/ /pubmed/1080792 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Clonal growth of hamster free alveolar cells in soft agar
title Clonal growth of hamster free alveolar cells in soft agar
title_full Clonal growth of hamster free alveolar cells in soft agar
title_fullStr Clonal growth of hamster free alveolar cells in soft agar
title_full_unstemmed Clonal growth of hamster free alveolar cells in soft agar
title_short Clonal growth of hamster free alveolar cells in soft agar
title_sort clonal growth of hamster free alveolar cells in soft agar
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2189945/
https://www.ncbi.nlm.nih.gov/pubmed/1080792

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