Separation of helper and suppressor T lymphocytes on a ficoll velocity sedimentation gradient

A 5-20% Ficoll velocity sedimentation gradient has been successfully applied to separate concanavalin A (Con A)-induced helper; and suppressor T cells. When titrated into a constant number of fresh normal spleen cells responding to sheep erythrocytes, cells from the top pool show stimulatory effects...

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Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1976
Materias:
Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2190179/
https://www.ncbi.nlm.nih.gov/pubmed/1083419
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description A 5-20% Ficoll velocity sedimentation gradient has been successfully applied to separate concanavalin A (Con A)-induced helper; and suppressor T cells. When titrated into a constant number of fresh normal spleen cells responding to sheep erythrocytes, cells from the top pool show stimulatory effects while those from the bottom pool show inhibitory activity. Both activities are found to be Con A dependent and anti-theta sensitive. We conclude that Con A-induced helper and suppressor T cells are distinct subpopulations and such separation will allow further characterization of these cell types.
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spelling pubmed-21901792008-04-17 Separation of helper and suppressor T lymphocytes on a ficoll velocity sedimentation gradient J Exp Med Articles A 5-20% Ficoll velocity sedimentation gradient has been successfully applied to separate concanavalin A (Con A)-induced helper; and suppressor T cells. When titrated into a constant number of fresh normal spleen cells responding to sheep erythrocytes, cells from the top pool show stimulatory effects while those from the bottom pool show inhibitory activity. Both activities are found to be Con A dependent and anti-theta sensitive. We conclude that Con A-induced helper and suppressor T cells are distinct subpopulations and such separation will allow further characterization of these cell types. The Rockefeller University Press 1976-05-01 /pmc/articles/PMC2190179/ /pubmed/1083419 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Separation of helper and suppressor T lymphocytes on a ficoll velocity sedimentation gradient
title Separation of helper and suppressor T lymphocytes on a ficoll velocity sedimentation gradient
title_full Separation of helper and suppressor T lymphocytes on a ficoll velocity sedimentation gradient
title_fullStr Separation of helper and suppressor T lymphocytes on a ficoll velocity sedimentation gradient
title_full_unstemmed Separation of helper and suppressor T lymphocytes on a ficoll velocity sedimentation gradient
title_short Separation of helper and suppressor T lymphocytes on a ficoll velocity sedimentation gradient
title_sort separation of helper and suppressor t lymphocytes on a ficoll velocity sedimentation gradient
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2190179/
https://www.ncbi.nlm.nih.gov/pubmed/1083419

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