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Mass isolation and culture of rat kupffer cells
Collagenase perfusion of the liver followed by pronase treatment of the cell suspension thus obtained gave a quantitative recovery of viable nonparenchymal liver cells (NPC). From these NPC, Kupffer (K) cells can be purified by attachment to tissue culture dishes. Tail vein injection of carbon 1-2 h...
Formato: | Texto |
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Lenguaje: | English |
Publicado: |
The Rockefeller University Press
1975
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2190515/ https://www.ncbi.nlm.nih.gov/pubmed/1090696 |
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