Cargando…

Inhibition of multiplication of Toxoplasma gondii by human monocytes exposed to T-lymphocyte products

The multiplication of Toxoplasma gondii was quantitated in human monocytes in vitro by phase-contrast microscopy. Toxoplasma multiplication was identical in monocytes from subjects byt was significantly inhibited in cells from both sources if the monocytes were preincubated with immune lymphocytes a...

Descripción completa

Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1975
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2190524/
https://www.ncbi.nlm.nih.gov/pubmed/1089746
_version_ 1782146824307474432
collection PubMed
description The multiplication of Toxoplasma gondii was quantitated in human monocytes in vitro by phase-contrast microscopy. Toxoplasma multiplication was identical in monocytes from subjects byt was significantly inhibited in cells from both sources if the monocytes were preincubated with immune lymphocytes and toxoplasma monocytes were preincubated with immune lymphocytes and toxoplasma antigen. Supernates prepared from toxoplasma-immune lymphocytes incubated with toxoplasma antigen were also effective in inducing in monocytes the capacity to inhibit toxoplasma multiplication. Supernative acitivty was evident after lymphocytes and antigen were incubated for as little as 15 min. The instruction of monocytes was also repid and reversible. Monocytes were fully induced to inhibit toxoplasma multiplication after a 2 h exposure to an active supernate, but they lost their inhibitory capacity on culture in vitro for 48 h in the absece of immune cells or their products. The lymphocytes particupating in the monocyte induction were identified as t cells. The in vitro stimulation of monocytes appeared to exhibit some specificity, since no inhibition of toxopreotein derivative and lymphocytes from tuberculin-positive subjects, concanavalin a-stimulated lymphocytes, or their supermates. Supernates which induced monocytes to inhibit toxoplasma multiplication did not influence parasite growth in HeLa cells.
format Text
id pubmed-2190524
institution National Center for Biotechnology Information
language English
publishDate 1975
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-21905242008-04-17 Inhibition of multiplication of Toxoplasma gondii by human monocytes exposed to T-lymphocyte products J Exp Med Articles The multiplication of Toxoplasma gondii was quantitated in human monocytes in vitro by phase-contrast microscopy. Toxoplasma multiplication was identical in monocytes from subjects byt was significantly inhibited in cells from both sources if the monocytes were preincubated with immune lymphocytes and toxoplasma monocytes were preincubated with immune lymphocytes and toxoplasma antigen. Supernates prepared from toxoplasma-immune lymphocytes incubated with toxoplasma antigen were also effective in inducing in monocytes the capacity to inhibit toxoplasma multiplication. Supernative acitivty was evident after lymphocytes and antigen were incubated for as little as 15 min. The instruction of monocytes was also repid and reversible. Monocytes were fully induced to inhibit toxoplasma multiplication after a 2 h exposure to an active supernate, but they lost their inhibitory capacity on culture in vitro for 48 h in the absece of immune cells or their products. The lymphocytes particupating in the monocyte induction were identified as t cells. The in vitro stimulation of monocytes appeared to exhibit some specificity, since no inhibition of toxopreotein derivative and lymphocytes from tuberculin-positive subjects, concanavalin a-stimulated lymphocytes, or their supermates. Supernates which induced monocytes to inhibit toxoplasma multiplication did not influence parasite growth in HeLa cells. The Rockefeller University Press 1975-02-01 /pmc/articles/PMC2190524/ /pubmed/1089746 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Inhibition of multiplication of Toxoplasma gondii by human monocytes exposed to T-lymphocyte products
title Inhibition of multiplication of Toxoplasma gondii by human monocytes exposed to T-lymphocyte products
title_full Inhibition of multiplication of Toxoplasma gondii by human monocytes exposed to T-lymphocyte products
title_fullStr Inhibition of multiplication of Toxoplasma gondii by human monocytes exposed to T-lymphocyte products
title_full_unstemmed Inhibition of multiplication of Toxoplasma gondii by human monocytes exposed to T-lymphocyte products
title_short Inhibition of multiplication of Toxoplasma gondii by human monocytes exposed to T-lymphocyte products
title_sort inhibition of multiplication of toxoplasma gondii by human monocytes exposed to t-lymphocyte products
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2190524/
https://www.ncbi.nlm.nih.gov/pubmed/1089746