Cargando…

Identification of an extracellular plasmin binding protein from nephritogenic streptococci

Examination of the extracellular products of nephritis(+) and nephritis(-) group A streptococci revealed the presence of a 46-kD protein secreted by nephritogenic strains that binds to human plasmin. Immunological data revealed that this protein, called nephritis plasmin binding protein (NPBP), is n...

Descripción completa

Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1993
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2191139/
https://www.ncbi.nlm.nih.gov/pubmed/8340765
_version_ 1782146934805364736
collection PubMed
description Examination of the extracellular products of nephritis(+) and nephritis(-) group A streptococci revealed the presence of a 46-kD protein secreted by nephritogenic strains that binds to human plasmin. Immunological data revealed that this protein, called nephritis plasmin binding protein (NPBP), is not related to group A streptokinase nor to a recently described streptococcal dehydrogenase protein. The binding of human plasmin to this protein can be blocked by epsilon-amino caproic acid, indicating the importance of lysine groups in the binding process. Mutanolysin extracts of cell walls from these nephritogenic strains probed with anti-NPBP antibody were negative for cell wall- bound NPBP. Serological data with acute poststreptococcal glomerulonephritis (APSGN) and acute rheumatic fever sera indicated that the protein reacts preferentially with APSGN sera. Amino acid sequence analysis and immunological reactivity suggest NPBP is the streptococcal pyrogenic exotoxin B precursor, also previously described as zymogen (streptococcal proteinase precursor). The secretion of both group A streptokinase and a secreted plasmin binding protein in the same nephritogenic strain raises an intriguing hypothesis of the mechanisms of action of this protein in APSGN.
format Text
id pubmed-2191139
institution National Center for Biotechnology Information
language English
publishDate 1993
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-21911392008-04-16 Identification of an extracellular plasmin binding protein from nephritogenic streptococci J Exp Med Articles Examination of the extracellular products of nephritis(+) and nephritis(-) group A streptococci revealed the presence of a 46-kD protein secreted by nephritogenic strains that binds to human plasmin. Immunological data revealed that this protein, called nephritis plasmin binding protein (NPBP), is not related to group A streptokinase nor to a recently described streptococcal dehydrogenase protein. The binding of human plasmin to this protein can be blocked by epsilon-amino caproic acid, indicating the importance of lysine groups in the binding process. Mutanolysin extracts of cell walls from these nephritogenic strains probed with anti-NPBP antibody were negative for cell wall- bound NPBP. Serological data with acute poststreptococcal glomerulonephritis (APSGN) and acute rheumatic fever sera indicated that the protein reacts preferentially with APSGN sera. Amino acid sequence analysis and immunological reactivity suggest NPBP is the streptococcal pyrogenic exotoxin B precursor, also previously described as zymogen (streptococcal proteinase precursor). The secretion of both group A streptokinase and a secreted plasmin binding protein in the same nephritogenic strain raises an intriguing hypothesis of the mechanisms of action of this protein in APSGN. The Rockefeller University Press 1993-08-01 /pmc/articles/PMC2191139/ /pubmed/8340765 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Identification of an extracellular plasmin binding protein from nephritogenic streptococci
title Identification of an extracellular plasmin binding protein from nephritogenic streptococci
title_full Identification of an extracellular plasmin binding protein from nephritogenic streptococci
title_fullStr Identification of an extracellular plasmin binding protein from nephritogenic streptococci
title_full_unstemmed Identification of an extracellular plasmin binding protein from nephritogenic streptococci
title_short Identification of an extracellular plasmin binding protein from nephritogenic streptococci
title_sort identification of an extracellular plasmin binding protein from nephritogenic streptococci
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2191139/
https://www.ncbi.nlm.nih.gov/pubmed/8340765