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Ly-49 mediates EL4 lymphoma adhesion to isolated class I major histocompatibility complex molecules
Ly-49 is a recently identified cell surface molecule expressed on a subpopulation of natural killer (NK) cells and certain T lymphomas. It has been suggested, based on gene transfection and antibody blocking studies, that Ly-49 is a negative regulator of NK lytic activity, possibly through an intera...
Formato: | Texto |
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Lenguaje: | English |
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The Rockefeller University Press
1994
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2191421/ https://www.ncbi.nlm.nih.gov/pubmed/8113669 |
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collection | PubMed |
description | Ly-49 is a recently identified cell surface molecule expressed on a subpopulation of natural killer (NK) cells and certain T lymphomas. It has been suggested, based on gene transfection and antibody blocking studies, that Ly-49 is a negative regulator of NK lytic activity, possibly through an interaction with target cell class I molecules. However, it has not been demonstrated that class I molecules indeed serve as ligands for Ly-49. We have found that T lymphomas expressing Ly-49 bind isolated class I major histocompatibility complex (MHC) molecules but not class II molecules immobilized on plastic. Adhesion to class I molecules was not found with T lymphomas lacking Ly-49 expression. The Ly-49 expressing EL4 lymphoma bound Dd, Dk, and Kb, but not Kd, Kk, or Db, thus demonstrating a restricted pattern of class I adhesion. The observed cell adhesion was class I density dependent, and binding to Dd and Dk was extensively inhibited by the A1 monoclonal antibody directed against Ly-49. These results provide direct evidence for Ly-49 serving as a receptor for a subset of class I MHC molecules. |
format | Text |
id | pubmed-2191421 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1994 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21914212008-04-16 Ly-49 mediates EL4 lymphoma adhesion to isolated class I major histocompatibility complex molecules J Exp Med Articles Ly-49 is a recently identified cell surface molecule expressed on a subpopulation of natural killer (NK) cells and certain T lymphomas. It has been suggested, based on gene transfection and antibody blocking studies, that Ly-49 is a negative regulator of NK lytic activity, possibly through an interaction with target cell class I molecules. However, it has not been demonstrated that class I molecules indeed serve as ligands for Ly-49. We have found that T lymphomas expressing Ly-49 bind isolated class I major histocompatibility complex (MHC) molecules but not class II molecules immobilized on plastic. Adhesion to class I molecules was not found with T lymphomas lacking Ly-49 expression. The Ly-49 expressing EL4 lymphoma bound Dd, Dk, and Kb, but not Kd, Kk, or Db, thus demonstrating a restricted pattern of class I adhesion. The observed cell adhesion was class I density dependent, and binding to Dd and Dk was extensively inhibited by the A1 monoclonal antibody directed against Ly-49. These results provide direct evidence for Ly-49 serving as a receptor for a subset of class I MHC molecules. The Rockefeller University Press 1994-03-01 /pmc/articles/PMC2191421/ /pubmed/8113669 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Articles Ly-49 mediates EL4 lymphoma adhesion to isolated class I major histocompatibility complex molecules |
title | Ly-49 mediates EL4 lymphoma adhesion to isolated class I major histocompatibility complex molecules |
title_full | Ly-49 mediates EL4 lymphoma adhesion to isolated class I major histocompatibility complex molecules |
title_fullStr | Ly-49 mediates EL4 lymphoma adhesion to isolated class I major histocompatibility complex molecules |
title_full_unstemmed | Ly-49 mediates EL4 lymphoma adhesion to isolated class I major histocompatibility complex molecules |
title_short | Ly-49 mediates EL4 lymphoma adhesion to isolated class I major histocompatibility complex molecules |
title_sort | ly-49 mediates el4 lymphoma adhesion to isolated class i major histocompatibility complex molecules |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2191421/ https://www.ncbi.nlm.nih.gov/pubmed/8113669 |