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IL-4 and IL-13 induce Lsk, a Csk-like tyrosine kinase, in human monocytes
Lsk is a protein tyrosine kinase with homology to the COOH-terminal Src kinase (Csk). Unlike Csk that is ubiquitously expressed, Lsk has limited tissue distribution. Here we have examined the expression and regulation of Lsk and Csk in peripheral human monocytes. We have found that Lsk mRNA and prot...
Formato: | Texto |
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Lenguaje: | English |
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The Rockefeller University Press
1994
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2191790/ https://www.ncbi.nlm.nih.gov/pubmed/7964512 |
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collection | PubMed |
description | Lsk is a protein tyrosine kinase with homology to the COOH-terminal Src kinase (Csk). Unlike Csk that is ubiquitously expressed, Lsk has limited tissue distribution. Here we have examined the expression and regulation of Lsk and Csk in peripheral human monocytes. We have found that Lsk mRNA and protein were not expressed in resting monocytes but were induced by treatment with interleukin 4 (IL-4) or IL-13 but not by interferon gamma (IFN-gamma) or IL-2. In fact, IFN-gamma, but not IL-2, efficiently blocked Lsk induction by IL-4 or IL-13. In contrast, Csk was constitutively present in human monocytes and was upregulated by IFN-gamma but not by IL-4 or IL-13. These results suggest that despite their structural similarities, Lsk and Csk may play distinct regulatory roles in monocyte functions elicited by cytokines, with Lsk functioning specifically within the context of a Th2-type immune response. |
format | Text |
id | pubmed-2191790 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1994 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21917902008-04-16 IL-4 and IL-13 induce Lsk, a Csk-like tyrosine kinase, in human monocytes J Exp Med Articles Lsk is a protein tyrosine kinase with homology to the COOH-terminal Src kinase (Csk). Unlike Csk that is ubiquitously expressed, Lsk has limited tissue distribution. Here we have examined the expression and regulation of Lsk and Csk in peripheral human monocytes. We have found that Lsk mRNA and protein were not expressed in resting monocytes but were induced by treatment with interleukin 4 (IL-4) or IL-13 but not by interferon gamma (IFN-gamma) or IL-2. In fact, IFN-gamma, but not IL-2, efficiently blocked Lsk induction by IL-4 or IL-13. In contrast, Csk was constitutively present in human monocytes and was upregulated by IFN-gamma but not by IL-4 or IL-13. These results suggest that despite their structural similarities, Lsk and Csk may play distinct regulatory roles in monocyte functions elicited by cytokines, with Lsk functioning specifically within the context of a Th2-type immune response. The Rockefeller University Press 1994-12-01 /pmc/articles/PMC2191790/ /pubmed/7964512 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Articles IL-4 and IL-13 induce Lsk, a Csk-like tyrosine kinase, in human monocytes |
title | IL-4 and IL-13 induce Lsk, a Csk-like tyrosine kinase, in human monocytes |
title_full | IL-4 and IL-13 induce Lsk, a Csk-like tyrosine kinase, in human monocytes |
title_fullStr | IL-4 and IL-13 induce Lsk, a Csk-like tyrosine kinase, in human monocytes |
title_full_unstemmed | IL-4 and IL-13 induce Lsk, a Csk-like tyrosine kinase, in human monocytes |
title_short | IL-4 and IL-13 induce Lsk, a Csk-like tyrosine kinase, in human monocytes |
title_sort | il-4 and il-13 induce lsk, a csk-like tyrosine kinase, in human monocytes |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2191790/ https://www.ncbi.nlm.nih.gov/pubmed/7964512 |