Cargando…
Peptide influences the folding and intracellular transport of free major histocompatibility complex class I heavy chains
Class I major histocompatibility complex molecules require both beta 2- microglobulin (beta 2m) and peptide for efficient intracellular transport. With the exception of H-2Db and Ld, class I heavy chains have not been detectable at the surface of cells lacking beta 2m. We show that properly conforme...
Formato: | Texto |
---|---|
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
1995
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2191915/ https://www.ncbi.nlm.nih.gov/pubmed/7869032 |
_version_ | 1782147117118128128 |
---|---|
collection | PubMed |
description | Class I major histocompatibility complex molecules require both beta 2- microglobulin (beta 2m) and peptide for efficient intracellular transport. With the exception of H-2Db and Ld, class I heavy chains have not been detectable at the surface of cells lacking beta 2m. We show that properly conformed class I heavy chains can be detected in a terminally glycosylated form indicative of cell surface expression in H- 2b, H-2d, and H-2s beta 2m-/- concanavalin A (Con A)-stimulated splenocytes incubated at reduced temperature. Furthermore, we demonstrate the presence of Kb molecules at the surface of beta 2m-/- cells cultured at 37 degrees C. The mode of assembly of class I molecules encompasses two major pathways: binding of peptide to preformed "empty" heterodimers, and binding of peptide to free heavy chains, followed by recruitment of beta 2m. In support of the existence of the latter pathway, we provide evidence for a role of peptide in intracellular transport of free class I heavy chains, through analysis of Con A-stimulated splenocytes from transporter associated with antigen processing 1 (TAP1)-/-, beta 2m-/-, and double-mutant TAP1/beta 2m-/- mice. |
format | Text |
id | pubmed-2191915 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1995 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21919152008-04-16 Peptide influences the folding and intracellular transport of free major histocompatibility complex class I heavy chains J Exp Med Articles Class I major histocompatibility complex molecules require both beta 2- microglobulin (beta 2m) and peptide for efficient intracellular transport. With the exception of H-2Db and Ld, class I heavy chains have not been detectable at the surface of cells lacking beta 2m. We show that properly conformed class I heavy chains can be detected in a terminally glycosylated form indicative of cell surface expression in H- 2b, H-2d, and H-2s beta 2m-/- concanavalin A (Con A)-stimulated splenocytes incubated at reduced temperature. Furthermore, we demonstrate the presence of Kb molecules at the surface of beta 2m-/- cells cultured at 37 degrees C. The mode of assembly of class I molecules encompasses two major pathways: binding of peptide to preformed "empty" heterodimers, and binding of peptide to free heavy chains, followed by recruitment of beta 2m. In support of the existence of the latter pathway, we provide evidence for a role of peptide in intracellular transport of free class I heavy chains, through analysis of Con A-stimulated splenocytes from transporter associated with antigen processing 1 (TAP1)-/-, beta 2m-/-, and double-mutant TAP1/beta 2m-/- mice. The Rockefeller University Press 1995-03-01 /pmc/articles/PMC2191915/ /pubmed/7869032 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Articles Peptide influences the folding and intracellular transport of free major histocompatibility complex class I heavy chains |
title | Peptide influences the folding and intracellular transport of free major histocompatibility complex class I heavy chains |
title_full | Peptide influences the folding and intracellular transport of free major histocompatibility complex class I heavy chains |
title_fullStr | Peptide influences the folding and intracellular transport of free major histocompatibility complex class I heavy chains |
title_full_unstemmed | Peptide influences the folding and intracellular transport of free major histocompatibility complex class I heavy chains |
title_short | Peptide influences the folding and intracellular transport of free major histocompatibility complex class I heavy chains |
title_sort | peptide influences the folding and intracellular transport of free major histocompatibility complex class i heavy chains |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2191915/ https://www.ncbi.nlm.nih.gov/pubmed/7869032 |