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Induction of G1 arrest by down-regulation of cyclin D3 in T cell hybridomas
The relationship between activation-induced growth inhibition and regulation of the cell cycle progression was investigated in T cell hybridomas by studying the function of the cell cycle-regulating genes such as G1 cyclins and their associated kinases. Activation of T cell hybridomas by anti-T cell...
Formato: | Texto |
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Lenguaje: | English |
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The Rockefeller University Press
1995
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2192146/ https://www.ncbi.nlm.nih.gov/pubmed/7629502 |
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collection | PubMed |
description | The relationship between activation-induced growth inhibition and regulation of the cell cycle progression was investigated in T cell hybridomas by studying the function of the cell cycle-regulating genes such as G1 cyclins and their associated kinases. Activation of T cell hybridomas by anti-T cell receptor antibody induces growth arrest at G1 phase of the cell cycle and subsequently results in activation-driven cell death. Rapid reduction of both messenger RNA and protein level of the cyclin D3 is accompanied by growth arrest upon activation. Although the residual cyclin D3 protein forms a complex with cdk4 protein, cyclin D3-dependent kinase activity is severely impaired. Stable transfectants engineered to express cyclin D3 override the growth arrest upon activation. These results imply that the activation signal through T cell receptor induces the down-regulation of cyclin D3 expression and cyclin D3-dependent kinase activity, leading to growth arrest in G1 phase of the cell cycle in T cells. |
format | Text |
id | pubmed-2192146 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1995 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21921462008-04-16 Induction of G1 arrest by down-regulation of cyclin D3 in T cell hybridomas J Exp Med Articles The relationship between activation-induced growth inhibition and regulation of the cell cycle progression was investigated in T cell hybridomas by studying the function of the cell cycle-regulating genes such as G1 cyclins and their associated kinases. Activation of T cell hybridomas by anti-T cell receptor antibody induces growth arrest at G1 phase of the cell cycle and subsequently results in activation-driven cell death. Rapid reduction of both messenger RNA and protein level of the cyclin D3 is accompanied by growth arrest upon activation. Although the residual cyclin D3 protein forms a complex with cdk4 protein, cyclin D3-dependent kinase activity is severely impaired. Stable transfectants engineered to express cyclin D3 override the growth arrest upon activation. These results imply that the activation signal through T cell receptor induces the down-regulation of cyclin D3 expression and cyclin D3-dependent kinase activity, leading to growth arrest in G1 phase of the cell cycle in T cells. The Rockefeller University Press 1995-08-01 /pmc/articles/PMC2192146/ /pubmed/7629502 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Articles Induction of G1 arrest by down-regulation of cyclin D3 in T cell hybridomas |
title | Induction of G1 arrest by down-regulation of cyclin D3 in T cell hybridomas |
title_full | Induction of G1 arrest by down-regulation of cyclin D3 in T cell hybridomas |
title_fullStr | Induction of G1 arrest by down-regulation of cyclin D3 in T cell hybridomas |
title_full_unstemmed | Induction of G1 arrest by down-regulation of cyclin D3 in T cell hybridomas |
title_short | Induction of G1 arrest by down-regulation of cyclin D3 in T cell hybridomas |
title_sort | induction of g1 arrest by down-regulation of cyclin d3 in t cell hybridomas |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2192146/ https://www.ncbi.nlm.nih.gov/pubmed/7629502 |