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Mice deficient in IL-1beta manifest impaired contact hypersensitivity to trinitrochlorobenzone

Mice rendered deficient in IL-1 beta by gene targeting in embryonic stem cells develop and grow normally in a protected laboratory environment. Endotoxin-stimulated peritoneal macrophages from IL-1beta- deficient mice showed normal synthesis and cellular release of IL- 1alpha after treatment with 5...

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Detalles Bibliográficos
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 1996
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2192516/
https://www.ncbi.nlm.nih.gov/pubmed/8666901
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collection PubMed
description Mice rendered deficient in IL-1 beta by gene targeting in embryonic stem cells develop and grow normally in a protected laboratory environment. Endotoxin-stimulated peritoneal macrophages from IL-1beta- deficient mice showed normal synthesis and cellular release of IL- 1alpha after treatment with 5 mM ATP demonstrating that IL-1beta is not necessary for expression and release of the IL-1alpha isoform. Mice deficient in IL-1beta showed unaltered sensitivity to endotoxic shock, with or without pretreatment with D-galactosamine. In contrast, IL- 1beta-deficient mice showed defective contact hypersensitivity responses to topically applied trinitrochlorobenzene (TNCB). This defect could be overcome either by application of very high doses of sensitizing antigen, or by local intradermal injection of recombinant IL-1beta immediately before antigen application. These data demonstrate an essential role for IL-1beta in contact hypersensitivity and suggest that IL-1beta acts early during the sensitization phase of response. They suggest an important role for IL-1beta in initiation of the host of response at the epidermal barrier.
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spelling pubmed-21925162008-04-16 Mice deficient in IL-1beta manifest impaired contact hypersensitivity to trinitrochlorobenzone J Exp Med Articles Mice rendered deficient in IL-1 beta by gene targeting in embryonic stem cells develop and grow normally in a protected laboratory environment. Endotoxin-stimulated peritoneal macrophages from IL-1beta- deficient mice showed normal synthesis and cellular release of IL- 1alpha after treatment with 5 mM ATP demonstrating that IL-1beta is not necessary for expression and release of the IL-1alpha isoform. Mice deficient in IL-1beta showed unaltered sensitivity to endotoxic shock, with or without pretreatment with D-galactosamine. In contrast, IL- 1beta-deficient mice showed defective contact hypersensitivity responses to topically applied trinitrochlorobenzene (TNCB). This defect could be overcome either by application of very high doses of sensitizing antigen, or by local intradermal injection of recombinant IL-1beta immediately before antigen application. These data demonstrate an essential role for IL-1beta in contact hypersensitivity and suggest that IL-1beta acts early during the sensitization phase of response. They suggest an important role for IL-1beta in initiation of the host of response at the epidermal barrier. The Rockefeller University Press 1996-04-01 /pmc/articles/PMC2192516/ /pubmed/8666901 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Articles
Mice deficient in IL-1beta manifest impaired contact hypersensitivity to trinitrochlorobenzone
title Mice deficient in IL-1beta manifest impaired contact hypersensitivity to trinitrochlorobenzone
title_full Mice deficient in IL-1beta manifest impaired contact hypersensitivity to trinitrochlorobenzone
title_fullStr Mice deficient in IL-1beta manifest impaired contact hypersensitivity to trinitrochlorobenzone
title_full_unstemmed Mice deficient in IL-1beta manifest impaired contact hypersensitivity to trinitrochlorobenzone
title_short Mice deficient in IL-1beta manifest impaired contact hypersensitivity to trinitrochlorobenzone
title_sort mice deficient in il-1beta manifest impaired contact hypersensitivity to trinitrochlorobenzone
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2192516/
https://www.ncbi.nlm.nih.gov/pubmed/8666901