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Fas Gene Mutation in the Progression of Adult T Cell Leukemia
Fas antigen (Apo-1/CD95) is an apoptosis-signaling cell surface receptor belonging to the tumor necrosis factor receptor superfamily. Adult T cell leukemia (ATL) cells express Fas antigen and show apoptosis after treatment with an anti-Fas monoclonal antibody. We established the ATL cell line KOB, w...
Autores principales: | , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
1999
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2193006/ https://www.ncbi.nlm.nih.gov/pubmed/10190897 |
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author | Maeda, Takahiro Yamada, Yasuaki Moriuchi, Ryozou Sugahara, Kazuyuki Tsuruda, Kazuto Joh, Tatsurou Atogami, Sunao Tsukasaki, Kunihiro Tomonaga, Masao Kamihira, Shimeru |
author_facet | Maeda, Takahiro Yamada, Yasuaki Moriuchi, Ryozou Sugahara, Kazuyuki Tsuruda, Kazuto Joh, Tatsurou Atogami, Sunao Tsukasaki, Kunihiro Tomonaga, Masao Kamihira, Shimeru |
author_sort | Maeda, Takahiro |
collection | PubMed |
description | Fas antigen (Apo-1/CD95) is an apoptosis-signaling cell surface receptor belonging to the tumor necrosis factor receptor superfamily. Adult T cell leukemia (ATL) cells express Fas antigen and show apoptosis after treatment with an anti-Fas monoclonal antibody. We established the ATL cell line KOB, which showed resistance to Fas-mediated apoptosis, and found that KOB expressed two forms of Fas mRNA, the normal form and a truncated form. The truncated transcript lacked 20 base pairs at exon 9, resulting in a frame shift and the generation of a premature stop codon at amino acid 239. The same mutation was detected in primary ascitic cells and peripheral blood cells. The mutation was not detected in lymph node cells, however, although all of the primary ATL cells were of the same clonal origin. A retroviral-mediated gene transfer of the truncated Fas to Jurkat cells rendered the cells resistant to Fas-mediated apoptosis, suggesting a dominant negative interference mechanism. These results indicate that an ATL subclone acquires a Fas mutation in the lymph nodes, enabling the subclone to escape from apoptosis mediated by the Fas/Fas ligand system and proliferate in the body. Mutation of the Fas gene may be one of the mechanisms underlying the progression of ATL. |
format | Text |
id | pubmed-2193006 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1999 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21930062008-04-16 Fas Gene Mutation in the Progression of Adult T Cell Leukemia Maeda, Takahiro Yamada, Yasuaki Moriuchi, Ryozou Sugahara, Kazuyuki Tsuruda, Kazuto Joh, Tatsurou Atogami, Sunao Tsukasaki, Kunihiro Tomonaga, Masao Kamihira, Shimeru J Exp Med Articles Fas antigen (Apo-1/CD95) is an apoptosis-signaling cell surface receptor belonging to the tumor necrosis factor receptor superfamily. Adult T cell leukemia (ATL) cells express Fas antigen and show apoptosis after treatment with an anti-Fas monoclonal antibody. We established the ATL cell line KOB, which showed resistance to Fas-mediated apoptosis, and found that KOB expressed two forms of Fas mRNA, the normal form and a truncated form. The truncated transcript lacked 20 base pairs at exon 9, resulting in a frame shift and the generation of a premature stop codon at amino acid 239. The same mutation was detected in primary ascitic cells and peripheral blood cells. The mutation was not detected in lymph node cells, however, although all of the primary ATL cells were of the same clonal origin. A retroviral-mediated gene transfer of the truncated Fas to Jurkat cells rendered the cells resistant to Fas-mediated apoptosis, suggesting a dominant negative interference mechanism. These results indicate that an ATL subclone acquires a Fas mutation in the lymph nodes, enabling the subclone to escape from apoptosis mediated by the Fas/Fas ligand system and proliferate in the body. Mutation of the Fas gene may be one of the mechanisms underlying the progression of ATL. The Rockefeller University Press 1999-04-05 /pmc/articles/PMC2193006/ /pubmed/10190897 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Articles Maeda, Takahiro Yamada, Yasuaki Moriuchi, Ryozou Sugahara, Kazuyuki Tsuruda, Kazuto Joh, Tatsurou Atogami, Sunao Tsukasaki, Kunihiro Tomonaga, Masao Kamihira, Shimeru Fas Gene Mutation in the Progression of Adult T Cell Leukemia |
title | Fas Gene Mutation in the Progression of Adult T Cell Leukemia |
title_full | Fas Gene Mutation in the Progression of Adult T Cell Leukemia |
title_fullStr | Fas Gene Mutation in the Progression of Adult T Cell Leukemia |
title_full_unstemmed | Fas Gene Mutation in the Progression of Adult T Cell Leukemia |
title_short | Fas Gene Mutation in the Progression of Adult T Cell Leukemia |
title_sort | fas gene mutation in the progression of adult t cell leukemia |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2193006/ https://www.ncbi.nlm.nih.gov/pubmed/10190897 |
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