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MD-2, a Molecule that Confers Lipopolysaccharide Responsiveness on Toll-like Receptor 4
Toll-like receptor 4 (TLR4) is a mammalian homologue of Drosophila Toll, a leucine-rich repeat molecule that can trigger innate responses against pathogens. The TLR4 gene has recently been shown to be mutated in C3H/HeJ and C57BL/10ScCr mice, both of which are low responders to lipopolysaccharide (L...
Autores principales: | , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
1999
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2193086/ https://www.ncbi.nlm.nih.gov/pubmed/10359581 |
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author | Shimazu, Rintaro Akashi, Sachiko Ogata, Hirotaka Nagai, Yoshinori Fukudome, Kenji Miyake, Kensuke Kimoto, Masao |
author_facet | Shimazu, Rintaro Akashi, Sachiko Ogata, Hirotaka Nagai, Yoshinori Fukudome, Kenji Miyake, Kensuke Kimoto, Masao |
author_sort | Shimazu, Rintaro |
collection | PubMed |
description | Toll-like receptor 4 (TLR4) is a mammalian homologue of Drosophila Toll, a leucine-rich repeat molecule that can trigger innate responses against pathogens. The TLR4 gene has recently been shown to be mutated in C3H/HeJ and C57BL/10ScCr mice, both of which are low responders to lipopolysaccharide (LPS). TLR4 may be a long-sought receptor for LPS. However, transfection of TLR4 does not confer LPS responsiveness on a recipient cell line, suggesting a requirement for an additional molecule. Here, we report that a novel molecule, MD-2, is requisite for LPS signaling of TLR4. MD-2 is physically associated with TLR4 on the cell surface and confers responsiveness to LPS. MD-2 is thus a link between TLR4 and LPS signaling. Identification of this new receptor complex has potential implications for understanding host defense, as well as pathophysiologic, mechanisms. |
format | Text |
id | pubmed-2193086 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1999 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21930862008-04-16 MD-2, a Molecule that Confers Lipopolysaccharide Responsiveness on Toll-like Receptor 4 Shimazu, Rintaro Akashi, Sachiko Ogata, Hirotaka Nagai, Yoshinori Fukudome, Kenji Miyake, Kensuke Kimoto, Masao J Exp Med Articles Toll-like receptor 4 (TLR4) is a mammalian homologue of Drosophila Toll, a leucine-rich repeat molecule that can trigger innate responses against pathogens. The TLR4 gene has recently been shown to be mutated in C3H/HeJ and C57BL/10ScCr mice, both of which are low responders to lipopolysaccharide (LPS). TLR4 may be a long-sought receptor for LPS. However, transfection of TLR4 does not confer LPS responsiveness on a recipient cell line, suggesting a requirement for an additional molecule. Here, we report that a novel molecule, MD-2, is requisite for LPS signaling of TLR4. MD-2 is physically associated with TLR4 on the cell surface and confers responsiveness to LPS. MD-2 is thus a link between TLR4 and LPS signaling. Identification of this new receptor complex has potential implications for understanding host defense, as well as pathophysiologic, mechanisms. The Rockefeller University Press 1999-06-07 /pmc/articles/PMC2193086/ /pubmed/10359581 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Articles Shimazu, Rintaro Akashi, Sachiko Ogata, Hirotaka Nagai, Yoshinori Fukudome, Kenji Miyake, Kensuke Kimoto, Masao MD-2, a Molecule that Confers Lipopolysaccharide Responsiveness on Toll-like Receptor 4 |
title | MD-2, a Molecule that Confers Lipopolysaccharide Responsiveness on Toll-like Receptor 4 |
title_full | MD-2, a Molecule that Confers Lipopolysaccharide Responsiveness on Toll-like Receptor 4 |
title_fullStr | MD-2, a Molecule that Confers Lipopolysaccharide Responsiveness on Toll-like Receptor 4 |
title_full_unstemmed | MD-2, a Molecule that Confers Lipopolysaccharide Responsiveness on Toll-like Receptor 4 |
title_short | MD-2, a Molecule that Confers Lipopolysaccharide Responsiveness on Toll-like Receptor 4 |
title_sort | md-2, a molecule that confers lipopolysaccharide responsiveness on toll-like receptor 4 |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2193086/ https://www.ncbi.nlm.nih.gov/pubmed/10359581 |
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