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Identification of a Human Follicular Dendritic Cell Molecule That Stimulates Germinal Center B Cell Growth
The initial interaction between B cells and follicular dendritic cells (FDCs) appears to be essential for germinal center (GC) formation. To identify molecules regulating this interaction, we generated FDC-staining monoclonal antibodies (mAbs) and screened them for their ability to block FDC-mediate...
Autores principales: | , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
2000
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2193124/ https://www.ncbi.nlm.nih.gov/pubmed/10727470 |
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author | Li, Li Zhang, Xin Kovacic, Sharlotte Long, Andrew J. Bourque, Karen Wood, Clive R. Choi, Yong Sung |
author_facet | Li, Li Zhang, Xin Kovacic, Sharlotte Long, Andrew J. Bourque, Karen Wood, Clive R. Choi, Yong Sung |
author_sort | Li, Li |
collection | PubMed |
description | The initial interaction between B cells and follicular dendritic cells (FDCs) appears to be essential for germinal center (GC) formation. To identify molecules regulating this interaction, we generated FDC-staining monoclonal antibodies (mAbs) and screened them for their ability to block FDC-mediated costimulation of growth and differentiation of CD40-stimulated B cells. Using one of the inhibitory mAbs, 8D6, we expression cloned the cDNA encoding the 8D6 antigen (Ag) from a human FDC line, HK. The 8D6 Ag is a novel protein of 282 amino acids that is expressed abundantly on FDCs. Monolayers of COS cells transiently transfected with the 8D6 Ag cDNA stimulate B cell growth. The mAb 8D6 blocks the costimulatory function completely. The inhibitory activity of the mAb 8D6 was demonstrated to be due to an inhibition of cell cycle progression of CD40 ligand–stimulated GC B cells. In addition, the mAb 8D6 inhibits the growth of a lymphoma of GC origin, L3055, which depends on FDCs or HK cells for its growth. These findings suggest that the primary function of FDCs in the GC is to stimulate B cell growth. An FDC signal molecule, 8D6 Ag, may be an important molecule to mediate this function. |
format | Text |
id | pubmed-2193124 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2000 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21931242008-04-16 Identification of a Human Follicular Dendritic Cell Molecule That Stimulates Germinal Center B Cell Growth Li, Li Zhang, Xin Kovacic, Sharlotte Long, Andrew J. Bourque, Karen Wood, Clive R. Choi, Yong Sung J Exp Med Brief Definitive Report The initial interaction between B cells and follicular dendritic cells (FDCs) appears to be essential for germinal center (GC) formation. To identify molecules regulating this interaction, we generated FDC-staining monoclonal antibodies (mAbs) and screened them for their ability to block FDC-mediated costimulation of growth and differentiation of CD40-stimulated B cells. Using one of the inhibitory mAbs, 8D6, we expression cloned the cDNA encoding the 8D6 antigen (Ag) from a human FDC line, HK. The 8D6 Ag is a novel protein of 282 amino acids that is expressed abundantly on FDCs. Monolayers of COS cells transiently transfected with the 8D6 Ag cDNA stimulate B cell growth. The mAb 8D6 blocks the costimulatory function completely. The inhibitory activity of the mAb 8D6 was demonstrated to be due to an inhibition of cell cycle progression of CD40 ligand–stimulated GC B cells. In addition, the mAb 8D6 inhibits the growth of a lymphoma of GC origin, L3055, which depends on FDCs or HK cells for its growth. These findings suggest that the primary function of FDCs in the GC is to stimulate B cell growth. An FDC signal molecule, 8D6 Ag, may be an important molecule to mediate this function. The Rockefeller University Press 2000-03-20 /pmc/articles/PMC2193124/ /pubmed/10727470 Text en © 2000 The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Brief Definitive Report Li, Li Zhang, Xin Kovacic, Sharlotte Long, Andrew J. Bourque, Karen Wood, Clive R. Choi, Yong Sung Identification of a Human Follicular Dendritic Cell Molecule That Stimulates Germinal Center B Cell Growth |
title | Identification of a Human Follicular Dendritic Cell Molecule That Stimulates Germinal Center B Cell Growth |
title_full | Identification of a Human Follicular Dendritic Cell Molecule That Stimulates Germinal Center B Cell Growth |
title_fullStr | Identification of a Human Follicular Dendritic Cell Molecule That Stimulates Germinal Center B Cell Growth |
title_full_unstemmed | Identification of a Human Follicular Dendritic Cell Molecule That Stimulates Germinal Center B Cell Growth |
title_short | Identification of a Human Follicular Dendritic Cell Molecule That Stimulates Germinal Center B Cell Growth |
title_sort | identification of a human follicular dendritic cell molecule that stimulates germinal center b cell growth |
topic | Brief Definitive Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2193124/ https://www.ncbi.nlm.nih.gov/pubmed/10727470 |
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