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Analysis of Immunoglobulin (Ig) Isotype Diversity and Igm/D Memory in the Response to Phenyl-Oxazolone
The distribution of immunoglobulin (Ig) isotypes within specific B cell clones in vivo after immunization is not well defined. Using an IgV(H)/CDR3- and isotype-specific reverse transcription polymerase chain reaction method, we have carried out a survey of the diversification of the isotype in a sp...
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
2000
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2193197/ https://www.ncbi.nlm.nih.gov/pubmed/10859344 |
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author | White, Harry Gray, David |
author_facet | White, Harry Gray, David |
author_sort | White, Harry |
collection | PubMed |
description | The distribution of immunoglobulin (Ig) isotypes within specific B cell clones in vivo after immunization is not well defined. Using an IgV(H)/CDR3- and isotype-specific reverse transcription polymerase chain reaction method, we have carried out a survey of the diversification of the isotype in a splenic response to phenyl-oxazolone (phOx) on a chicken serum albumin carrier. The phOx-specific V(H) (V(H)Ox-1 with specific CDR3 motif) is associated with all of the heavy chains (μ, δ, α, γ, and ε) after simple immunization with antigen in alum. The kinetics of expression of each isotype are distinct and reproducible. Focusing mainly on the expression of secretory Ig transcripts, IgM, IgG1, and IgE are found after priming, whereas IgD and IgA appear after boosting. Secretory IgD transcripts are found reproducibly at moderate levels and may, therefore, contribute significantly to the secreted Ig response in mice. Most crucially, we find enhanced levels of secretory IgM/V(H)Ox-1 transcripts (with ‘phOx-specific’ CDR3) after boosting, strongly indicating the existence of IgM memory cells that give rise to an enhanced specific IgM secretion in the secondary response. |
format | Text |
id | pubmed-2193197 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2000 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21931972008-04-16 Analysis of Immunoglobulin (Ig) Isotype Diversity and Igm/D Memory in the Response to Phenyl-Oxazolone White, Harry Gray, David J Exp Med Original Article The distribution of immunoglobulin (Ig) isotypes within specific B cell clones in vivo after immunization is not well defined. Using an IgV(H)/CDR3- and isotype-specific reverse transcription polymerase chain reaction method, we have carried out a survey of the diversification of the isotype in a splenic response to phenyl-oxazolone (phOx) on a chicken serum albumin carrier. The phOx-specific V(H) (V(H)Ox-1 with specific CDR3 motif) is associated with all of the heavy chains (μ, δ, α, γ, and ε) after simple immunization with antigen in alum. The kinetics of expression of each isotype are distinct and reproducible. Focusing mainly on the expression of secretory Ig transcripts, IgM, IgG1, and IgE are found after priming, whereas IgD and IgA appear after boosting. Secretory IgD transcripts are found reproducibly at moderate levels and may, therefore, contribute significantly to the secreted Ig response in mice. Most crucially, we find enhanced levels of secretory IgM/V(H)Ox-1 transcripts (with ‘phOx-specific’ CDR3) after boosting, strongly indicating the existence of IgM memory cells that give rise to an enhanced specific IgM secretion in the secondary response. The Rockefeller University Press 2000-06-19 /pmc/articles/PMC2193197/ /pubmed/10859344 Text en © 2000 The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Original Article White, Harry Gray, David Analysis of Immunoglobulin (Ig) Isotype Diversity and Igm/D Memory in the Response to Phenyl-Oxazolone |
title | Analysis of Immunoglobulin (Ig) Isotype Diversity and Igm/D Memory in the Response to Phenyl-Oxazolone |
title_full | Analysis of Immunoglobulin (Ig) Isotype Diversity and Igm/D Memory in the Response to Phenyl-Oxazolone |
title_fullStr | Analysis of Immunoglobulin (Ig) Isotype Diversity and Igm/D Memory in the Response to Phenyl-Oxazolone |
title_full_unstemmed | Analysis of Immunoglobulin (Ig) Isotype Diversity and Igm/D Memory in the Response to Phenyl-Oxazolone |
title_short | Analysis of Immunoglobulin (Ig) Isotype Diversity and Igm/D Memory in the Response to Phenyl-Oxazolone |
title_sort | analysis of immunoglobulin (ig) isotype diversity and igm/d memory in the response to phenyl-oxazolone |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2193197/ https://www.ncbi.nlm.nih.gov/pubmed/10859344 |
work_keys_str_mv | AT whiteharry analysisofimmunoglobulinigisotypediversityandigmdmemoryintheresponsetophenyloxazolone AT graydavid analysisofimmunoglobulinigisotypediversityandigmdmemoryintheresponsetophenyloxazolone |