Cargando…
Macrophage Inflammatory Protein 3α Is Expressed at Inflamed Epithelial Surfaces and Is the Most Potent Chemokine Known in Attracting Langerhans Cell Precursors
Dendritic cells (DCs) form a network comprising different populations that initiate and differentially regulate immune responses. Langerhans cells (LCs) represent a unique population of DCs colonizing epithelium, and we present here observations suggesting that macrophage inflammatory protein (MIP)-...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2000
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2193271/ https://www.ncbi.nlm.nih.gov/pubmed/10974036 |
_version_ | 1782147431478067200 |
---|---|
author | Dieu-Nosjean, Marie-Caroline Massacrier, Catherine Homey, Bernhard Vanbervliet, Béatrice Pin, Jean-Jacques Vicari, Alain Lebecque, Serge Dezutter-Dambuyant, Colette Schmitt, Daniel Zlotnik, Albert Caux, Christophe |
author_facet | Dieu-Nosjean, Marie-Caroline Massacrier, Catherine Homey, Bernhard Vanbervliet, Béatrice Pin, Jean-Jacques Vicari, Alain Lebecque, Serge Dezutter-Dambuyant, Colette Schmitt, Daniel Zlotnik, Albert Caux, Christophe |
author_sort | Dieu-Nosjean, Marie-Caroline |
collection | PubMed |
description | Dendritic cells (DCs) form a network comprising different populations that initiate and differentially regulate immune responses. Langerhans cells (LCs) represent a unique population of DCs colonizing epithelium, and we present here observations suggesting that macrophage inflammatory protein (MIP)-3α plays a central role in LC precursor recruitment into the epithelium during inflammation. (a) Among DC populations, MIP-3α was the most potent chemokine inducing the selective migration of in vitro–generated CD34(+) hematopoietic progenitor cell–derived LC precursors and skin LCs in accordance with the restricted MIP-3α receptor (CC chemokine receptor 6) expression to these cells. (b) MIP-3α was mainly produced by epithelial cells, and the migration of LC precursors induced by the supernatant of activated skin keratinocytes was completely blocked with an antibody against MIP-3α. (c) In vivo, MIP-3α was selectively produced at sites of inflammation as illustrated in tonsils and lesional psoriatic skin where MIP-3α upregulation appeared associated with an increase in LC turnover. (d) Finally, the secretion of MIP-3α was strongly upregulated by cells of epithelial origin after inflammatory stimuli (interleukin 1β plus tumor necrosis factor α) or T cell signals. Results of this study suggest a major role of MIP-3α in epithelial colonization by LCs under inflammatory conditions and immune disorders, and might open new ways to control epithelial immunity. |
format | Text |
id | pubmed-2193271 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2000 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-21932712008-04-16 Macrophage Inflammatory Protein 3α Is Expressed at Inflamed Epithelial Surfaces and Is the Most Potent Chemokine Known in Attracting Langerhans Cell Precursors Dieu-Nosjean, Marie-Caroline Massacrier, Catherine Homey, Bernhard Vanbervliet, Béatrice Pin, Jean-Jacques Vicari, Alain Lebecque, Serge Dezutter-Dambuyant, Colette Schmitt, Daniel Zlotnik, Albert Caux, Christophe J Exp Med Original Article Dendritic cells (DCs) form a network comprising different populations that initiate and differentially regulate immune responses. Langerhans cells (LCs) represent a unique population of DCs colonizing epithelium, and we present here observations suggesting that macrophage inflammatory protein (MIP)-3α plays a central role in LC precursor recruitment into the epithelium during inflammation. (a) Among DC populations, MIP-3α was the most potent chemokine inducing the selective migration of in vitro–generated CD34(+) hematopoietic progenitor cell–derived LC precursors and skin LCs in accordance with the restricted MIP-3α receptor (CC chemokine receptor 6) expression to these cells. (b) MIP-3α was mainly produced by epithelial cells, and the migration of LC precursors induced by the supernatant of activated skin keratinocytes was completely blocked with an antibody against MIP-3α. (c) In vivo, MIP-3α was selectively produced at sites of inflammation as illustrated in tonsils and lesional psoriatic skin where MIP-3α upregulation appeared associated with an increase in LC turnover. (d) Finally, the secretion of MIP-3α was strongly upregulated by cells of epithelial origin after inflammatory stimuli (interleukin 1β plus tumor necrosis factor α) or T cell signals. Results of this study suggest a major role of MIP-3α in epithelial colonization by LCs under inflammatory conditions and immune disorders, and might open new ways to control epithelial immunity. The Rockefeller University Press 2000-09-05 /pmc/articles/PMC2193271/ /pubmed/10974036 Text en © 2000 The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Original Article Dieu-Nosjean, Marie-Caroline Massacrier, Catherine Homey, Bernhard Vanbervliet, Béatrice Pin, Jean-Jacques Vicari, Alain Lebecque, Serge Dezutter-Dambuyant, Colette Schmitt, Daniel Zlotnik, Albert Caux, Christophe Macrophage Inflammatory Protein 3α Is Expressed at Inflamed Epithelial Surfaces and Is the Most Potent Chemokine Known in Attracting Langerhans Cell Precursors |
title | Macrophage Inflammatory Protein 3α Is Expressed at Inflamed Epithelial Surfaces and Is the Most Potent Chemokine Known in Attracting Langerhans Cell Precursors |
title_full | Macrophage Inflammatory Protein 3α Is Expressed at Inflamed Epithelial Surfaces and Is the Most Potent Chemokine Known in Attracting Langerhans Cell Precursors |
title_fullStr | Macrophage Inflammatory Protein 3α Is Expressed at Inflamed Epithelial Surfaces and Is the Most Potent Chemokine Known in Attracting Langerhans Cell Precursors |
title_full_unstemmed | Macrophage Inflammatory Protein 3α Is Expressed at Inflamed Epithelial Surfaces and Is the Most Potent Chemokine Known in Attracting Langerhans Cell Precursors |
title_short | Macrophage Inflammatory Protein 3α Is Expressed at Inflamed Epithelial Surfaces and Is the Most Potent Chemokine Known in Attracting Langerhans Cell Precursors |
title_sort | macrophage inflammatory protein 3α is expressed at inflamed epithelial surfaces and is the most potent chemokine known in attracting langerhans cell precursors |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2193271/ https://www.ncbi.nlm.nih.gov/pubmed/10974036 |
work_keys_str_mv | AT dieunosjeanmariecaroline macrophageinflammatoryprotein3aisexpressedatinflamedepithelialsurfacesandisthemostpotentchemokineknowninattractinglangerhanscellprecursors AT massacriercatherine macrophageinflammatoryprotein3aisexpressedatinflamedepithelialsurfacesandisthemostpotentchemokineknowninattractinglangerhanscellprecursors AT homeybernhard macrophageinflammatoryprotein3aisexpressedatinflamedepithelialsurfacesandisthemostpotentchemokineknowninattractinglangerhanscellprecursors AT vanbervlietbeatrice macrophageinflammatoryprotein3aisexpressedatinflamedepithelialsurfacesandisthemostpotentchemokineknowninattractinglangerhanscellprecursors AT pinjeanjacques macrophageinflammatoryprotein3aisexpressedatinflamedepithelialsurfacesandisthemostpotentchemokineknowninattractinglangerhanscellprecursors AT vicarialain macrophageinflammatoryprotein3aisexpressedatinflamedepithelialsurfacesandisthemostpotentchemokineknowninattractinglangerhanscellprecursors AT lebecqueserge macrophageinflammatoryprotein3aisexpressedatinflamedepithelialsurfacesandisthemostpotentchemokineknowninattractinglangerhanscellprecursors AT dezutterdambuyantcolette macrophageinflammatoryprotein3aisexpressedatinflamedepithelialsurfacesandisthemostpotentchemokineknowninattractinglangerhanscellprecursors AT schmittdaniel macrophageinflammatoryprotein3aisexpressedatinflamedepithelialsurfacesandisthemostpotentchemokineknowninattractinglangerhanscellprecursors AT zlotnikalbert macrophageinflammatoryprotein3aisexpressedatinflamedepithelialsurfacesandisthemostpotentchemokineknowninattractinglangerhanscellprecursors AT cauxchristophe macrophageinflammatoryprotein3aisexpressedatinflamedepithelialsurfacesandisthemostpotentchemokineknowninattractinglangerhanscellprecursors |