Molecular Cloning and Biological Characterization of a Novel Murine Lymphoid Growth Factor

Using a bioassay consisting of the proliferation of a murine B cell line, a cDNA of a gene whose product supports the growth of that cell line was isolated from a thymic stromal cell line. This factor, termed thymic stromal lymphopoietin (TSLP), is a protein of 140 amino acids. The gene encoding TSL...

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Detalles Bibliográficos
Autores principales: Sims, John E., Williams, Douglas E., Morrissey, Philip J., Garka, Kirsten, Foxworthe, Diane, Price, Virginia, Friend, Sherree L., Farr, Andrew, Bedell, Mary A., Jenkins, Nancy A., Copeland, Neal G., Grabstein, Kenneth, Paxton, Raymond J.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2000
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2193273/
https://www.ncbi.nlm.nih.gov/pubmed/10974033
Descripción
Sumario:Using a bioassay consisting of the proliferation of a murine B cell line, a cDNA of a gene whose product supports the growth of that cell line was isolated from a thymic stromal cell line. This factor, termed thymic stromal lymphopoietin (TSLP), is a protein of 140 amino acids. The gene encoding TSLP was mapped to murine chromosome 18. Purified recombinant TSLP supported the growth of pre-B cell colonies in vitro, but had no myelopoietic activity. TSLP had comitogenic activity for fetal thymocytes, but was not as potent as interleukin 7 in lobe submersion cultures. Injection of TSLP into neonatal mice induced the expansion of B220(+)BP-1(+) pre-B cells.

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