Cloning of the Murine Thymic Stromal Lymphopoietin (Tslp) Receptor: Formation of a Functional Heteromeric Complex Requires Interleukin 7 Receptor

The cellular receptor for murine thymic stromal lymphopoietin (TSLP) was detected in a variety of murine, but not human myelomonocytic cell lines by radioligand binding. cDNA clones encoding the receptor were isolated from a murine T helper cell cDNA library. TSLP receptor (TSLPR) is a member of the...

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Detalles Bibliográficos
Autores principales: Park, Linda S., Martin, Unja, Garka, Kirsten, Gliniak, Brian, Di Santo, James P., Muller, Werner, Largaespada, David A., Copeland, Neal G., Jenkins, Nancy A., Farr, Andrew G., Ziegler, Steven F., Morrissey, Philip J., Paxton, Raymond, Sims, John E.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2000
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2193276/
https://www.ncbi.nlm.nih.gov/pubmed/10974032
Descripción
Sumario:The cellular receptor for murine thymic stromal lymphopoietin (TSLP) was detected in a variety of murine, but not human myelomonocytic cell lines by radioligand binding. cDNA clones encoding the receptor were isolated from a murine T helper cell cDNA library. TSLP receptor (TSLPR) is a member of the hematopoietin receptor family. Transfection of TSLPR cDNA resulted in only low affinity binding. Cotransfection of the interleukin 7 (IL-7)Rα chain cDNA resulted in conversion to high affinity binding. TSLP did not activate cells from IL-7Rα(−/)− mice, but did activate cells from γc(−/)− mice. Thus, the functional TSLPR requires the IL-7Rα chain, but not the γc chain for signaling.

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