Cloning of the Murine Thymic Stromal Lymphopoietin (Tslp) Receptor: Formation of a Functional Heteromeric Complex Requires Interleukin 7 Receptor

The cellular receptor for murine thymic stromal lymphopoietin (TSLP) was detected in a variety of murine, but not human myelomonocytic cell lines by radioligand binding. cDNA clones encoding the receptor were isolated from a murine T helper cell cDNA library. TSLP receptor (TSLPR) is a member of the...

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Autores principales: Park, Linda S., Martin, Unja, Garka, Kirsten, Gliniak, Brian, Di Santo, James P., Muller, Werner, Largaespada, David A., Copeland, Neal G., Jenkins, Nancy A., Farr, Andrew G., Ziegler, Steven F., Morrissey, Philip J., Paxton, Raymond, Sims, John E.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2000
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2193276/
https://www.ncbi.nlm.nih.gov/pubmed/10974032
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author Park, Linda S.
Martin, Unja
Garka, Kirsten
Gliniak, Brian
Di Santo, James P.
Muller, Werner
Largaespada, David A.
Copeland, Neal G.
Jenkins, Nancy A.
Farr, Andrew G.
Ziegler, Steven F.
Morrissey, Philip J.
Paxton, Raymond
Sims, John E.
author_facet Park, Linda S.
Martin, Unja
Garka, Kirsten
Gliniak, Brian
Di Santo, James P.
Muller, Werner
Largaespada, David A.
Copeland, Neal G.
Jenkins, Nancy A.
Farr, Andrew G.
Ziegler, Steven F.
Morrissey, Philip J.
Paxton, Raymond
Sims, John E.
author_sort Park, Linda S.
collection PubMed
description The cellular receptor for murine thymic stromal lymphopoietin (TSLP) was detected in a variety of murine, but not human myelomonocytic cell lines by radioligand binding. cDNA clones encoding the receptor were isolated from a murine T helper cell cDNA library. TSLP receptor (TSLPR) is a member of the hematopoietin receptor family. Transfection of TSLPR cDNA resulted in only low affinity binding. Cotransfection of the interleukin 7 (IL-7)Rα chain cDNA resulted in conversion to high affinity binding. TSLP did not activate cells from IL-7Rα(−/)− mice, but did activate cells from γc(−/)− mice. Thus, the functional TSLPR requires the IL-7Rα chain, but not the γc chain for signaling.
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spelling pubmed-21932762008-04-16 Cloning of the Murine Thymic Stromal Lymphopoietin (Tslp) Receptor: Formation of a Functional Heteromeric Complex Requires Interleukin 7 Receptor Park, Linda S. Martin, Unja Garka, Kirsten Gliniak, Brian Di Santo, James P. Muller, Werner Largaespada, David A. Copeland, Neal G. Jenkins, Nancy A. Farr, Andrew G. Ziegler, Steven F. Morrissey, Philip J. Paxton, Raymond Sims, John E. J Exp Med Original Article The cellular receptor for murine thymic stromal lymphopoietin (TSLP) was detected in a variety of murine, but not human myelomonocytic cell lines by radioligand binding. cDNA clones encoding the receptor were isolated from a murine T helper cell cDNA library. TSLP receptor (TSLPR) is a member of the hematopoietin receptor family. Transfection of TSLPR cDNA resulted in only low affinity binding. Cotransfection of the interleukin 7 (IL-7)Rα chain cDNA resulted in conversion to high affinity binding. TSLP did not activate cells from IL-7Rα(−/)− mice, but did activate cells from γc(−/)− mice. Thus, the functional TSLPR requires the IL-7Rα chain, but not the γc chain for signaling. The Rockefeller University Press 2000-09-05 /pmc/articles/PMC2193276/ /pubmed/10974032 Text en © 2000 The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Original Article
Park, Linda S.
Martin, Unja
Garka, Kirsten
Gliniak, Brian
Di Santo, James P.
Muller, Werner
Largaespada, David A.
Copeland, Neal G.
Jenkins, Nancy A.
Farr, Andrew G.
Ziegler, Steven F.
Morrissey, Philip J.
Paxton, Raymond
Sims, John E.
Cloning of the Murine Thymic Stromal Lymphopoietin (Tslp) Receptor: Formation of a Functional Heteromeric Complex Requires Interleukin 7 Receptor
title Cloning of the Murine Thymic Stromal Lymphopoietin (Tslp) Receptor: Formation of a Functional Heteromeric Complex Requires Interleukin 7 Receptor
title_full Cloning of the Murine Thymic Stromal Lymphopoietin (Tslp) Receptor: Formation of a Functional Heteromeric Complex Requires Interleukin 7 Receptor
title_fullStr Cloning of the Murine Thymic Stromal Lymphopoietin (Tslp) Receptor: Formation of a Functional Heteromeric Complex Requires Interleukin 7 Receptor
title_full_unstemmed Cloning of the Murine Thymic Stromal Lymphopoietin (Tslp) Receptor: Formation of a Functional Heteromeric Complex Requires Interleukin 7 Receptor
title_short Cloning of the Murine Thymic Stromal Lymphopoietin (Tslp) Receptor: Formation of a Functional Heteromeric Complex Requires Interleukin 7 Receptor
title_sort cloning of the murine thymic stromal lymphopoietin (tslp) receptor: formation of a functional heteromeric complex requires interleukin 7 receptor
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2193276/
https://www.ncbi.nlm.nih.gov/pubmed/10974032
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