Cargando…

Antigen-Specific T Helper Cell Function: Differential Cytokine Expression in Primary and Memory Responses

Distinguishing between the development of functional potential in antigen-specific T helper (Th) cells and the delivery of these specialized functions in vivo has been difficult to resolve. Here, we quantify the frequency of cytokine-producing cells within the primary and memory B10.BR Th cell respo...

Descripción completa

Detalles Bibliográficos
Autores principales: Panus, Joanne Fanelli, McHeyzer-Williams, Louise J., McHeyzer-Williams, Michael G.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2000
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2193351/
https://www.ncbi.nlm.nih.gov/pubmed/11067879
_version_ 1782147450400669696
author Panus, Joanne Fanelli
McHeyzer-Williams, Louise J.
McHeyzer-Williams, Michael G.
author_facet Panus, Joanne Fanelli
McHeyzer-Williams, Louise J.
McHeyzer-Williams, Michael G.
author_sort Panus, Joanne Fanelli
collection PubMed
description Distinguishing between the development of functional potential in antigen-specific T helper (Th) cells and the delivery of these specialized functions in vivo has been difficult to resolve. Here, we quantify the frequency of cytokine-producing cells within the primary and memory B10.BR Th cell response to pigeon cytochrome c (PCC). In vitro analysis of acquired functional potential indicated no Th1/Th2 cytokine polarity at the peak of the primary response with surprisingly little evidence for the selective preservation of interleukin (IL)-2, tumor necrosis factor (TNF)-α, IL-4, and interferon (IFN)-γ potentials into the memory compartment. However, the expression of these functional potentials appears tightly regulated in vivo. The staggered appearance of primary response cytokines directly ex vivo contrasts markedly with their rapid coordinate expression in the memory response. Frequencies of IL-2–, TNF-α–, IFN-γ–, and IL-10–expressing memory responders increased over their primary response counterparts, but were still markedly lower than revealed in vitro. IL-4–, IFN-γ–, and IL-10–expressing Th cells remained at low but stable frequencies over the first 6 d of the memory response. Analysis of T cell receptor β chain sequences of IL-4– and TNF-α–expressing PCC-specific Th cells provides evidence for early functional commitment among clonal progeny. These data indicate that the development of functional potential is a consequence of initial antigen experience, but delivery of specialized functions is differentially regulated in primary and memory immune responses.
format Text
id pubmed-2193351
institution National Center for Biotechnology Information
language English
publishDate 2000
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-21933512008-04-16 Antigen-Specific T Helper Cell Function: Differential Cytokine Expression in Primary and Memory Responses Panus, Joanne Fanelli McHeyzer-Williams, Louise J. McHeyzer-Williams, Michael G. J Exp Med Original Article Distinguishing between the development of functional potential in antigen-specific T helper (Th) cells and the delivery of these specialized functions in vivo has been difficult to resolve. Here, we quantify the frequency of cytokine-producing cells within the primary and memory B10.BR Th cell response to pigeon cytochrome c (PCC). In vitro analysis of acquired functional potential indicated no Th1/Th2 cytokine polarity at the peak of the primary response with surprisingly little evidence for the selective preservation of interleukin (IL)-2, tumor necrosis factor (TNF)-α, IL-4, and interferon (IFN)-γ potentials into the memory compartment. However, the expression of these functional potentials appears tightly regulated in vivo. The staggered appearance of primary response cytokines directly ex vivo contrasts markedly with their rapid coordinate expression in the memory response. Frequencies of IL-2–, TNF-α–, IFN-γ–, and IL-10–expressing memory responders increased over their primary response counterparts, but were still markedly lower than revealed in vitro. IL-4–, IFN-γ–, and IL-10–expressing Th cells remained at low but stable frequencies over the first 6 d of the memory response. Analysis of T cell receptor β chain sequences of IL-4– and TNF-α–expressing PCC-specific Th cells provides evidence for early functional commitment among clonal progeny. These data indicate that the development of functional potential is a consequence of initial antigen experience, but delivery of specialized functions is differentially regulated in primary and memory immune responses. The Rockefeller University Press 2000-11-06 /pmc/articles/PMC2193351/ /pubmed/11067879 Text en © 2000 The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Original Article
Panus, Joanne Fanelli
McHeyzer-Williams, Louise J.
McHeyzer-Williams, Michael G.
Antigen-Specific T Helper Cell Function: Differential Cytokine Expression in Primary and Memory Responses
title Antigen-Specific T Helper Cell Function: Differential Cytokine Expression in Primary and Memory Responses
title_full Antigen-Specific T Helper Cell Function: Differential Cytokine Expression in Primary and Memory Responses
title_fullStr Antigen-Specific T Helper Cell Function: Differential Cytokine Expression in Primary and Memory Responses
title_full_unstemmed Antigen-Specific T Helper Cell Function: Differential Cytokine Expression in Primary and Memory Responses
title_short Antigen-Specific T Helper Cell Function: Differential Cytokine Expression in Primary and Memory Responses
title_sort antigen-specific t helper cell function: differential cytokine expression in primary and memory responses
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2193351/
https://www.ncbi.nlm.nih.gov/pubmed/11067879
work_keys_str_mv AT panusjoannefanelli antigenspecificthelpercellfunctiondifferentialcytokineexpressioninprimaryandmemoryresponses
AT mcheyzerwilliamslouisej antigenspecificthelpercellfunctiondifferentialcytokineexpressioninprimaryandmemoryresponses
AT mcheyzerwilliamsmichaelg antigenspecificthelpercellfunctiondifferentialcytokineexpressioninprimaryandmemoryresponses